摘要
目的探讨肝炎肝硬化患者淋巴细胞亚群及抗原HLA-DR表达的临床意义。方法肝炎肝硬化患者66例分为脾未切除38例(A1组)和脾切除28例(A2组),以慢性乙型肝炎46例(B组)和健康者22例(C组)为对照。用流式细胞术检测外周血淋巴细胞各亚群及HLA-DR抗原。结果A2组外周血CD3+、CD4+及CD8+T细胞分别为(41.59±6.79)%、(22.64±6.87)%和(19.16±7.28)%,显著低于B组和C组(P<0.01);CD19+B细胞为(27.63±9.15)%,显著高于B组(P<0.05)和C组(P<0.01);HLA-DR抗原在CD3+、CD4+及CD8+T细胞上的表达为(25.42±5.51)%、(22.36±4.90)%和(28.53±8.35)%,显著低于B组(P<0.05或P<0.01)。结论肝炎肝硬化患者存在明显的细胞及体液免疫功能紊乱,主要表现为CD3+、CD4+、CD8+T细胞数量减少和细胞活性下降,以肝硬化脾切除患者尤为明显。
Objective To explore the expression and significance of lymphocyte subsets and HLA-DR antigen in patients with liver cirrhosis secondary to chronic hepatitis B（CHB）.MethodsSixty-six CHB patients were divided into 2 groups of cirrhosis without splenectomy（group A1,38 cases） and cirrhosis with splenectomy（group A2,28 cases）.Another 46 CHB patients（group CHB） and 22 healthy persons（group C） were taken as the controls.Lymphocyte subsets and the percentage of HLA-DR antigen in peripheral blood were detected by flow cytometry.Results The levels of CD3＋,CD4＋ and CD8＋ were （41.59 ±6.79）%,（22.64±6.87）% and （19.16±7.28）%,respectively in group A2,which were lower than those in groups of CHB and C（P0.01）.The percentage of CD19＋ was （27.63±9.15）% in group A2,which was higher than that group C（P0.01） and group CHB（P0.05）.The expressions of HLA-DR on CD3＋,CD4＋ and CD8＋ were （25.42±5.51）%,（22.36±4.90）% and （28.53±8.35）%,respectively in group A2,which were lower than those in group CHB（P0.05 or P0.01）.Conclusion Cell-mediated immune and humoral immunity of patients with cirrhosis are imbalanced,especially in the patients with splenectomy.The expressions of CD3＋,CD4＋ and CD8＋ and activity of T cell are decreased significantly in CHB patients with cirrhosis.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第6期628-630,共3页
Jiangsu Medical Journal
