摘要
肺泡巨噬细胞在吞噬异物和细菌过程中,或在酵母多糖等刺激后,能产生多种反应氧类(ROS),化学发光(CL)强度直接反映ROS的量,ROS不仅有强烈的杀菌功能,而且与免疫、衰老、炎症、癌症,类风湿性关节炎、缺血等有关,因而PAM的化学发光研究对探讨某些疾病发病机制,治疗将有一定意义。本文介绍了肺灌洗液(BAL)获取方法,肺泡巨噬细胞悬液的制备及化学发光测定方法,并对实验条件进行初步探讨。并应用化学发光方法测定64例肺泡巨噬细胞化学发光,其中正常人10例,患者54例,其中特发性肺纤维化、肺癌、肺炎,PAM的自发发光及刺激发光都显著高于正常对照组(P<0.01),哮喘、胸膜炎、结缔组织病与正常对照组也有区别(P<0.05),支扩、肺结核,结节病与正常对照组无统计学差异(P>0.05)。
A Iuminol-enhanced chemiluminescence (CL) assay was used to measure lightelaborated by pulmonary alveolar macrophages (PAMs) from 10 healthy normal sub-jects and 54 patients with different kinds of lung disease. Chemiluminescence assaywas performed at ambient temperature usiug a LKB 1250 photometer. Our resultsdemonstrated that substantially greater CL was produced by PAMs from patientswith idiopathic pulmonary fibrosis, lung cancer and pneumonia (P<0.01), and PAMsfrom patients with asthma, pleurisy and collagen diseases had higher CL (P<0.05),but no differences were noted between normal subjects and patients with brochiectasis,pulmonary tuberculosis and sarcoidosis. Increased CL by PAMs from patients withlung diseases may reflect phagocyte activation and large release of reactive oxygenspecies by PAMs in their disease process.
出处
《天津医药》
CAS
1992年第12期731-733,共3页
Tianjin Medical Journal
关键词
化学发光
肺泡
巨噬细胞
肺灌洗液
chemiluescence (CL)
pulmonary alveolar macrophage (PAM)
bronchial alveolar lavage (BAL)
zymosan
