摘要
本文通过分别以细胞计数和细胞蛋白作为基准，对肺部急性染酸大鼠肺粒细胞及正常大鼠肺巨噬细胞中总脂含量的比较研究，探讨了以细胞蛋白含量作为肺灌洗液细胞中成份测定基准的可能性和必要性。结果表明，当以细胞计数作为基准时，肺巨噬细胞中总脂含量为粒细胞的１．３２倍，而当以细胞蛋白作为基准时，则仅为２．５７倍，说明以细胞蛋白作为肺灌洗液细胞中成份测定的基准比用细胞计数或细胞浓度更为精确。
The necessity to take cellular protein
asstandard for the BAL ccllular content deter-mination were studied
by means of a compara-tive study of the total lipids content in
BALPMN issolated from rats intratracheally admi-nistrated with 2ml 0.
01mol/L HCl and inBAL pulmonary macrophages obtained fromnormal rats,
either cellular concentration orcellular protein was taken as the st
andard. The results showed that the total lipids inmacrophage was 2.
57-folds to that of PMNwhen the cellular protein was taken as
stan-dard, while only 1.32-folds in case of cellularconcentration as
standard. This suggested thatthe cellular protein would be more
accuratethan cellular concentration fOr the determina-tion of BAL
celldar contents.
出处
《职业医学》
1994年第1期2-4,共3页
China Occupational Medicine
基金
世界卫生组织(WHO)资助课题
关键词
肺灌洗液
细胞蛋白
测定基准
bronchoalveolar lavage, cellu-lar protein,
assay standard
