摘要
运用响应面分析法(中心组合设计)对干酪乳杆菌(Lactobacilluscasei)LB1 11生产L 乳酸的培养基C/N进行了研究,同时研究了有机氮源(蛋白胨、玉米浆和酵母膏)和无机氮源(NH4Cl和(NH4)2HPO4)对L 乳酸产量的影响,并进行了葡萄糖、蛋白胨、NH4Cl和(NH4)2HPO4四因素的响应面分析实验.结果表明,适宜的培养基C/N(质量比)为12∶1～13∶1,培养基组成:葡萄糖120.13g/L,蛋白胨19.342g/L,NH4Cl4g/L,(NH4)2HPO42.010g/L,L 乳酸产量可达到103g/L.优化后的培养基适合于对干酪乳杆菌进行代谢网络分析.
A detailed response surface methodology for L-(+)-lactic acid production by lactobacillus casei was carried out to optimize the C/N ratio of the fermentation medium using the experimental design. The first study tested two factors(organic nitrogen sources and inorganic nitrogen sources)and one medium point was performed. The second factorial design was based on surface response with four factors (glucose concentration, peptone concentration, NH_(4)Cl concentration, (NH_(4))_(2)HPO_(4) concentration). The statistical analysis of the data obtained from the factorial study showed that a C/N ratio of 12～13 and the glucose concentration of 120.13 (g/L), peptone concentration of 19.342 g/L,NH_(4)Cl concentration of 4 g/L, and (NH_(4))_(2)HPO_(4) concentration of 2.010 g/L were the optimal condition, with L-(+)-lactic acid concentration as high as 103 g/L obtained.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第6期55-58,63,共5页
Journal of Wuxi University of Light Industry
