摘要
目的 探索严重烧伤后小鼠脾脏T淋巴细胞功能改变及IL - 2、IL - 10分泌规律 ,并从T淋巴细胞活化跨膜信号转导途径寻找导致其改变的原因。 方法 检测T淋巴细胞表面抗原受体TCRα/ β ,辅助刺激分子CD2 8及参与跨膜信号转导的G蛋白、蛋白酪氨酸激酶 (PTK)、蛋白激酶C(PKC)的活性改变 ,观察伤后不同时相T淋巴细胞增殖转化功能及IL - 2、IL - 10分泌活性 ,分析各信号转导分子改变与T淋巴细胞功能活性改变之间的关系。 结果 烧伤后T细胞膜表面分子TCRα/β、CD2 8表达阳性率降低 ,GTPase活性和膜PTK活性均受抑 ,但于伤后 16 8h恢复 ,转膜PKC活性出现先降低后升高的双相改变 ,且与IL - 10水平密切相关。结论 烧伤后T细胞膜表面分子TCRα/ β、CD2 8和跨膜信号转导酶活性的改变是导致伤后IL - 2分泌降低、T细胞功能受抑和IL -
Objective ToexplorethepostburnfunctionalchangeinsplenicTlymhocytesandthesecretory rulesofIL - 2andIL - 10inseverelyscaldedmice ,andtolookfortheexplanationofthechangebymeansofT lymphocytetransmembranesignaltransdnctionstudy . Methods ThechangesintheantigenreceptorsonTcells (TCRα/β) ,assistingstimulatingmolecule(CD2 8)andtheactivitiesofGTPase ,proteintyrosinekinase(PTK)and proteinkinaseC (PKC)thatparticipatedinthetransmembranesignaltransductionweredetected ,soastoobserve theproliferationandtransformationfunctionofTcellsatdifferentpostburnperiodsandthesecretionofIL - 2and IL - 10 .Furthermore ,thechangesineverysignaltransductionmoleculeswereanalyzedwiththeconsiderationof theirrelationshipwiththechangeinTcellfunctionactivities. Results Thepostburnpositiveratesofthe expressionsofTCRα/βandCD2 8onTcellmembranedecreased .ThepostburnGTPaseactivityandmembranePTK activitywereallsuppressedbutrecoveredat16 8postburnhours .MembranePKCactivityexhibitedadual -phase change (increasefollowingdecrease) ,whichwascloselyrelatedtoIL - 10level. Conclusion Thepostburn changesinTCRα/β,CD2 8onTcellmembranemoleculesandtransmembranesignaltransductionenzymeswere importantfactorscontributingtothedecreaseinIL - 2secretion ,suppressedTcellfunctionandthedual - directionalchangesinIL - 10secretion .
出处
《中华烧伤杂志》
CAS
CSCD
2000年第6期352-354,共3页
Chinese Journal of Burns
基金
国家自然科学基金资助项目 !(395 0 0 14 9)
