摘要
目的：通过体内和体外实验，验证雷公藤内酯醇是否具有预防同种异体移植术后急性排异反应的作用。方法：以环孢霉素Ａ（ＣｓＡ）为阳性对照，采用同种异体的皮肤移植和肾移植。结果：雷公藤内酯醇能够①明显延长同种异体移植的皮肤和肾的存活时间，雷公藤内酯醇剂量为２００μｇ／（ｋｇ·ｄ）时，二者的平均存活时间为１７２和２９１ｄ（对照组分别为９８和９２，Ｐ＜００１）；②联合使用亚有效剂量的雷公藤内酯醇与ＣｓＡ时，能够明显地延长同种异体移植的皮肤和肾脏的存活时间；③移植肾病理检查发现，雷公藤内酯醇治疗后，移植肾组织中病理损伤程度明显轻于未治疗组。结论：本研究表明，雷公藤内酯醇具有明显地抗同种异体急性排异反应的作用。
OBJECTIVE Triptolide is a potent element in Tripterygium Wilfordii Hook f. that has been proved efficient in the treatment of glomerulonephritis by affecting immunological events of the diseases. In this study, Triptolide is used in renal allograft model in a dose response protocol to observe the effects on renal allograft rejection in rat. METHODOLOGY Renal grafting was performed between Wistar rat(RT y) as recipient and Lou/MN(RT l) as donor. Animals treated with different dose of triptolide(T1 group, 50 μg/kg·d -1 ; T2 group, 100 μg/kg·d -1 ; T3 group, 200 μg/kg·d -1 ) 14 days before transplantation were compare both to rats not treated with triptolide(T0 group) and to rats treated with cyclosporine A (at different doses, 5 mg/kg·d -1 , 10 mg/kg·d -1 and 20 mg/kg·d -1 )after transplantation(CsA group). In each group, four rats were sacrificed at the 7th day and four at the 14th day after transplantation for pathological studies. A synergistic study were carried out in 9 rats by combining triptolide with CsA (4 treated with Triptolide 50 μg/kg·d -1 + CsA 5 mg/kg·d -1 ; and 5 with Triptolide 100 μg/kg·d -1 +CsA 5 mg/kg·d -1 ) RESULTS (1) The mean survival time of renal allografts in T0( n =5), T1( n =5),T2( n =4), T3( n =7) groups and CsA(dosage at 20 mg/kg·d -1 ) were 9.2, 12.0, 16.2, 29.4 and 26.7days respectively. (2) In the pathological study, grafts from rats in T3 group at day 7 and day 14 showed no evidence of acute allograft rejection; while grafts from rats in T2 group showed mild interstitial mononuclear cell infiltration and slight evidence of tubulitis and arteritis at day 7, and features of moderate rejections were observed in grafts at day 14. (3)At the dosage ranging from 50 μg/kg to 200 μg/kg started 14 days before transplantation, triptolide significantly prolonged graft survivals compared to controls. When triptolide and CsA were used together, a synergistic effect was observed. CONCLUSION Triptolide is effective in preventing renal allograft rejection in a dose dependent manner and is synergistic with CsA .
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1997年第3期214-220,共7页
Chinese Journal of Nephrology,Dialysis & Transplantation
