摘要
目的:观察在兔耳创面愈合前后使用IFN-γ对瘢痕形成的影响并检测瘢痕组织中PTK活性的变化,探讨二者间的关系。方法:在兔耳腹侧伤口愈合前(A组)和后(C组)以及连续使用IFN-γ(B组),观察瘢痕形成情况并测定正常兔耳皮肤、创面上皮化时(12-14天)及其后3天、7天、14天和21天时瘢痕组织中PTK活性变化。结果:A、B和C组瘢痕组织中PTK活性变化规律与对照侧相同:上皮化后活性均逐渐升高,和正常皮肤组织比较(P<0.001),21天时基本恢复正常皮肤的水平;各组的处理侧在3天和7天时以及A、B组上皮化时PTK活性高于对照侧(P<0.05)。IFN-γ能抑制瘢痕增生,以A组和B组作用最强(P<0.05)。结论:创伤愈合后瘢痕组织中PTK活性升高提示PTK介导的信号可能参与瘢痕改建过程。不同时期使用IFN-γ能进一步活化PTK并显著抑制瘢痕增生意味着IFN-γ的抑制作用可能经活化PTK介导。
Objective:To study signal effect of protein tyrosine kinase(PTK)in IFN -γinhibiting the wound healing and the scar for-mation of rabbit ear.Methods:IFN -γwas used on the wound of rabbit ear til l healing(group A),till 3d of post -epithelialization(group B)and only 1d -3d of post -epithelializ ation(group C ).The PTK activity of the tissue from 0d,3d,7d,14d and 21d of post -ep-ithelization was measured by phosphorus incorporation.Results:The PTK activity increased signific antly gradually at the post -epithe-lization comparing with the normal s kin(P<0.001)and restored to the level of normal sk in about 21d of the post -epithelization.The PTK activity were higher at epithelization,3d and 7d of post -epitheliza tion at the side of using IFN -γcomparing with the control ones in all groups(P<0.05).Moreover,IFN -γinhibited the hypertrophic scar formation.Conclusion:It was suggested that PTK -mediated signal pathway might participate in the scar remolding and mediate IFN -γinhibitory effects on formation of t he hypertrophic scar.
出处
《中国美容医学》
CAS
2002年第6期524-526,共3页
Chinese Journal of Aesthetic Medicine
