摘要
用皮下注射醋酸可的松法诱导大鼠卡氏肺孢子虫肺炎(PCP)模型,成功率20％。对PCP模型大鼠(n=6)进行诊断采样,结果:肺病理学检查卡氏肺孢子虫(PC)检出率为100％(6/6)。用经支气管肺活检(TBLB)法临床诊断1例早期PCP。支气管肺泡灌洗液(BALF)涂片和肺组织印片的姬姆萨染色,可见PC囊内小体及滋养体。改进的哥氏银染适用于肺组织切片,包囊壁浓染呈黑色,易于识别。对于免疫功能低下怀疑为PCP患者,应尽早作TBLB和(或)BAL检查,可以达到早期诊断。
A rat model of Pneumocystis oarinii pneumonia (PCP) was established by fluboutaneous injections of cortisone acetate. The total positive provocation rate was 20%, resulting in 6 wistar with pop. Various lung histopathological specimens were studied, all were positive (6/6). One clinical case of early POP was diag- nosed successfully by transbronchial lung biopsy (TBLB). Giemsa stain was used on smears obtained from bronchial alveolar lavage fluids (BALF) and on lung imprints, intraoystio bodies and trophozoites of Pneumooystis carinii were revealed apparently. Modified Gomori-Grocott's methenamine silver nitrate stain is suitable for pulmonary histopathological slides, as oyst walls of Pneumooystis carinii stained black and could be easily recognized. TBLB and/or BAL should be performed as early as possible in immunocompromised patients with suspected PCP, for an early diagnosis of PCP.
出处
《上海医科大学学报》
CSCD
1992年第3期173-177,共5页
Journal of Fudan University(Medical Science)
关键词
动物模型
PCP
肺炎
pneumoeystis oarinii pneumonia
animal model
identification method
