摘要
目的 :观察血管内皮生长因子 (vascularendothelialgrowthfactor ,VEGF )在大鼠正畸牙齿移动中牙周组织内的表达和分布 ,探讨VEGF在正畸牙齿移动中的作用机制。方法 :3 0只雄性SD大鼠 ,随机分为 6组 ,每组 5只 ,分 1、3、7、14、2 1d正畸加力组和正常对照组。采用免疫组织化学方法检测牙周组织VEGF的表达 ,并采用计算机图像分析方法对各组VEGF的表达强度进行半定量分析。结果 :VEGF在正畸加力组大鼠牙周组织中的表达明显强于正常对照组 ,加力 3d组的压力侧VEGF表达增强 ,其张力侧也有VEGF的表达 (略低于压力侧 ) ,7、14d为表达高峰期 (P <0 .0 1) ,2 1d组 ,VEGF表达有所减弱。VEGF的表达位于血管内皮细胞、成纤维细胞、成骨细胞和破骨细胞胞浆内。结论 :正畸牙齿移动中VEGF表达的变化 ,说明VEGF参与了牙齿移动过程中的牙周组织改建 ,可能对正畸牙齿移动修复具有重要意义。
Objective:To observe the expression and distribution of vascular endothelial growth factor (VEGF) in periodontal tissues, and analyze the role of VEGF in orthodontic tooth movement. Method:30 male Sprague-Dawley rats were randomly divided into six groups, of one control group and five experimental groups, each group consisted of 5 rats. In the experimental groups, 50g mesial force was applied to pull the right maxillary first molar in the rats. The experimental rats were killed after 1、3、7、14、21 days, respectively. Using immunohistochemical method to localize and examine the expression of VEGF in decalcified alveodental connective tissues. Result:The expression of VEGF in the experimental group enhance apparently compared with that in the control group. 3 days after the experimental tooth movement, the expression of VEGF was found on both the pressure and tension side of the alveolar bone. On 7 and 14 day ,it reached the peak level(P<0.01). In 21 days group, the expression of VEGF declined. VEGF was expressed in the cytoplasm of vascular endothelial cells、 fibroblasts、 osteoblasts and osteoclasts. Conclusion:VEGF was highly expressed during orthodontic tooth movement, suggesting that VEGF may participate in the tissue remodeling during orthodontic tooth movement and especially played a more important role in orthodontic bone formation.
出处
《临床口腔医学杂志》
2003年第11期659-661,共3页
Journal of Clinical Stomatology
