摘要
目的 了解乙型肝炎病毒(HBV)感染者血清HBV-DNA含量、HBV血清学标志物及肝功能指标(ALT、AST)的相互关系及意义。方法 分别采用荧光定量聚合酶链反应(PCR)技术和ELISA检测211例乙型肝炎患者血清HBV-DNA含量和HBV标志物,并与肝功能指标进行比较。结果 Pre S1抗原在HBeAg阳性模式中的阳性率(66.2％)高于HBeAg阴性模式中的阳性率(22.1％)(P<0.01);HBeAg和Pre S1抗原的阳性率随HBV-DNA拷贝数的升高而升高;HBV-DNA(-)组与HBV-DNA(+)组的ALT和AST差异显著(P<0.01),而HBV-DNA(+)组之间HBV-DNA拷贝数与ALT和AST无相关性。结论 Pre S1抗原、HBeAg和HBV-DNA含量三者彼此相关,均反映了HBV复制活性,其中HBV-DNA是最理想的指标;HBV-DNA能用于粗略判断肝功能是否正常。
Objective To know the relationships among the concentrations of serum HBV-DNA, serum HBV markers and the functional index of liver (ALT、AST) in patients with hepatitis B infection, and its clinical significance. Methods Serum HBV-DNA and HBV markers in 211 patients with hepatitis B were detected respectively by fluorescence quantitative polymerase chain reaction (PCR) technology and ELISA ,and compared with the functional index of the liver. Results Pre S1 antigen positive rato(66. 2%) in HBeAg positive model was higher than that(22. 1%) in HBeAg negative model (P<0. 01). The positive rates of HBeAg and Pre S1 antigen increased with the levels of HBV-DNA. ALT and AST had significant difference between HBV-DNA( - ) group and HBV-DNA(+) group. But among HBV-DNA( + ) group,HBV-DNA copies had no relation with ALT and AST. Conclusion Pre S1 antigen,HBeAg and HBV-DNA levels,which reflect the replication activity of HBV,are correlative with another. HBV-DNA is the best index,and can be roughly used to judge whether the function of liver is normal.
出处
《现代检验医学杂志》
CAS
2003年第5期31-32,共2页
Journal of Modern Laboratory Medicine
