摘要
目的探讨血清HBV DNA水平与HBV血清标志物(HBVM)的相关性。方法对1248例临床血清标本采用荧光定量PCR法进行HBV DNA检测,同时采用ELISA法进行乙肝免疫学对比检测。结果血清HBV DNA水平与HBV血清标志物的表现模式有关。以HBs Ag(+)、HBe Ag(+)、HBc Ab(+)模式患者的HBV DNA阳性检出率最高,为97.3%(499/513),DNA平均拷贝数为8.41×10^(7)/ml;其次为HBs Ag(+)、HBe Ab(+)、HBc Ab(+)模式,HBV DNA阳性检出率为49.4%(133/269),平均拷贝数为2.33×10^(5)/ml,均显著高于其他HBVM模式的患者。结论HBe Ag和HBV DNA有明显的相关性。定量检测HBV DNA能真实反映HBV复制情况,为乙型肝炎的诊断及疗效评价提供客观依据。
Objective To explore the relation between the quantity of HBV DNA and HBV serum markers.Meth-ods Contents of HBV DNA and the HBV serum markers were determined by FQ-PCR technique and ELISA respectivelyin 1248 serum samples.Results Contents of HBV DNA were related to HBV serum markers.The positive rate of HBVDNA was 97.3%(499/513)in the model of HBsAg(+),HBeAg(+)plus HBcAb(+),the highest among all models,andwith an average copy number of 8.41×10^(7)copies/ml.The positive rate of HBV DNA was 49.4%(133/269 samples)inthe model of HBsAg(+),HBeAb(+)plus HBcAb(+)with an average copy number of 2.33×10^(8)copies/ml.The HBVDNA positive rate of these two models were significantly higher than the others(P<0.01).Conclusion There is a signifi-cant correlation between HBeAg and HBV DNA.HBV DNA quantitative determination could reflect the replication of HBVand provide objective evidences for diagnosis and treatment monitoring of hepatitis B.
作者
陈学民
凡任芝
CHENXue-min;FAN Ren-zhi(Anhui Province Center for Clinical Laboratory Sciences,Hefei 230001)
出处
《临床输血与检验》
CAS
2005年第1期18-19,共2页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
乙型肝炎病毒
定量PCR
血清标志物
Hepatitis B virus
Quantitative polymerase chain reaction
Serum markers
