摘要
以错配聚合酶链反应产物作限制片段长度多态性（ＲＦＬＰ）分析，检测５４例经肝活检确诊的慢性乙型肝炎血清前Ｃ区变异株，总检出率为６６．７％；在慢性活动性肝炎中的检出率（８０．０％），明显高于慢性迁延性肝炎（４６．７％）；在乙型肝炎病毒ｅ抗原（ＨＢｅＡｇ）阳性和抗－ＨＢｅ（＋）中分别为４１．２％和６３．３％；在抗－ＨＢｅ（＋）且丙氨酸转氦酶正常血清中检出率８２．４％。前Ｃ区变异株可在病程中出现亦可消失。结果提示，前Ｃ区变异株与原型株的混合感染广泛存在于慢性乙型肝炎病毒感染者，其发生可能与机体免疫压力有关。
The point mutation in the precore region of hepatitis B viral genome( nt83 ) was tested with the method of mispairing PCR-RFLP in 54 chronic hepatitis B patients all confirmed by liver biopsy.The over all detection rate of pre-C mutation was 66.7% and the detection rate of pre-C mutation in chronic active hepatitis patients was as high as 80.0%,being significantly higher than that in chronic persistent hepatitis(46.7%). The detection rate of pre-C mutation was 41.2% in hepatitis B e antigen (HBeAg)positive group and 63.3%in anti-HBe(+)group respectively. The detection rate in anti-HBe(+)patients with normal alanine transan1inase activity was as high as 82.4%,During the period of follow up it was found that pre-C mutation may appear and disappear or may persist continually. The results suggested that pre-C mutation was extremely common in chronic hepatitis B virus(HBV)infection. The coexistence of the mu- tant type and wild type in the patients may be considered as the natural course of HBV chronic infection. Further study on the cause and effect relation between pre-C mutation and genesis of chronic active hepati- tis and liver cirrhosis is urgently needed.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1995年第4期232-234,共3页
Chinese Journal of Internal Medicine
