摘要
目的 :观察还原型谷胱甘肽 (GSH)对实验性慢性肝损伤的保护作用。方法 :用四氯化碳致大鼠慢性肝损伤模型 ,GSH分别采用灌胃及腹腔注射两个途径给药 ,剂量分别为 1.2 5 g/kg、0 .5g/kg、0 .2 g/kg及 0 .4 g/kg、0 .1g/kg、0 .0 2 5 g/kg ,实验结束时采血检测ALT、AST、白蛋白等 ,并解剖动物分离肝脏 ,作肝病理形态、纤维化分级及Ⅰ型胶原免疫组化观察。结果 :GSH灌胃及腹腔注射给药组的ALT、AST水平较模型组明显为低。病理形态观察发现 ,GSH能使肝细胞肿胀及脂肪变性程度明显减轻 ,抑制纤维组织的增生及Ⅰ型胶原的表达。结论 :GSH对四氯化碳所致的慢性肝损伤有明显的保护作用 ,为其临床应用提供了药理学基础。
Objective:To observe the prevention of glutathione(GSH)against chronic liver injury in rat.Methods:The chronic liver injury model was made by intracutaneous injection of carbon tetrachloride twice a week in rats for 24 times.From the 8th week after the carbon tetrachloride injection,GSH was given by oral or peritoneal administration once a day for 60 days.The animals were sacrificed at the end of the experiment.The serum level of ALT,AST were measured.The liver tissue was studied with histopathological or immunohistochemical assay.Results:GSH markly decreased the serum level of ALT,AST and improved liver tissue inflammation and lessend the liver fibrosis in histopathology compared with the model group.In the immunohistochemical stain,the deposition of type Ⅰ collagen of liver tissue significantly decreased in GSH treatment group compared with the model group.Conclusion:GSH can protect live from injury induced by toxic material.
出处
《重庆医科大学学报》
CAS
CSCD
2003年第5期592-594,635,共4页
Journal of Chongqing Medical University
