摘要
目的 :探讨内耳组织线粒体DNA(mtDNA)大片段缺失与老年性聋发病的关系。方法 :取Wistar大鼠按年龄分为A组 (青年鼠 ,4月龄 )和B组 (老年鼠 ,>2 4月龄 ) ,每组 15只。测试听性脑干反应 (ABR) ,应用聚合酶链反应 (PCR)技术对大鼠内耳膜迷路组织mtDNA 4 834bp缺失片段进行检测。结果 :A、B组大鼠ABR反应阈均值分别为 :(4 0 .0 0± 4 .6 6 )dBpeSPL、(6 4 .79± 10 .88)dBpeSPL ,二者之间差异有显著性意义 (P <0 .0 1)。大鼠内耳组织mtDNA检测发现 :A组大鼠未检测到 4 834bp缺失 ;B组大鼠有 9只存在 4 834bp缺失。 结论 :老年大鼠内耳膜迷路组织存在mtDNA片段缺失 ,这种缺失与老年性聋有关 。
Objective:By observing the relationship between the fragment deletion of mitichondrial DNA (mt DNA) of inner ear in rat and the hearing loss to investigate the possible role of mt DNA deletion in presbycusis. Method: Thirty wistar rats were divided into group A (young rat,4 months, n=15) and group B (old rat,24～30 months, n=15) according to the age.The threshold of the auditory brainstem response (ABR) was tested. The inner ear membranous labyrinth tissue was harvested and the mtDNA was amplified to identify 4834 bp deletion by PCR technique.Result:The mean of ABR thresholds in group B ( 64.79± 10.88dB peSPL)was significantly higher than that in group A ( 40.00± 4.66 dB peSPL)(P< 0.01). In group B, 9 of the 15 rats demonstrates the mtDNA 4834 bp deletion,while no deletion was detected in group A.Conclusion:Deletion of mtDNA of inner ear tissue in old rat is found.The mtDNA deletion is associated with the presbycusis and may be one of the causes of presbycusis.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2003年第11期678-680,共3页
Journal of Clinical Otorhinolaryngology
基金
国家自然科学基金资助项目 (No .396 70 781)
