摘要
目的 :研究成人咽喉部良、恶性病变与人乳头状瘤病毒 (HPV)感染的关系。方法 :应用聚合酶链反应 (PCR)和斑点杂交技术 ,对 5 5例咽喉不同病变的新鲜组织标本进行 HPV6,1 1 ,1 6,1 8,33共 5型HPV- DNA感染的检测。结果 :在咽乳头状瘤组 HPV感染率为 6 0 % (6 / 1 0 ) ,喉乳头状瘤组为 70 % (7/1 0 ) ,喉鳞状上皮非典型增生组为 2 0 % (1 / 5 ) ,声带息肉组为 2 0 % (1 / 5 ) ,喉癌组为 2 0 % (1 / 5 ) ,声带小结组为 0 (0 / 1 0 )。HPV- DNA型别分布在咽喉良性病变中以 HPV6,1 1 型为主 ,喉癌中以 HPV1 6为主。结论 :本地区成人咽喉良、恶性病变的发生与 HPV感染密切相关。PCR结合核酸斑点杂交法检测 HPV,具有灵敏性高、特异性强的优点 ,值得推广应用。
The HPV (human papilloma viruses) 6,11,16,18 and 33 DNA were determined by methods of polymerase chan reaction (PCR) and dot blot hybridization in 55 specimens of pharyngeal and laryngeal diseases.There were 10 pharyngeal papilloma,10 laryngeal papilloma,5 atypical epithelial hyperplasia,15 vocal cords polyps,10 vocal cords nodule and 5 laryngeal carcinoma,which all were diagnosed by pathology.Results showed that the positive rate of HPV DNA was 60 0%(6/10) in pharyngeal papilloma,20 0%(1/5) in atypical epithelial hyperplasia,20 0%(3/5) in vocal cords polyps,20 0%(1/5) in laryngeal carcinoma and 0 0%(0/10) in vocal cords nodule.The total positive rate of HPV DNA was 32 7%(18/55) in 55 specimens.Its found that the HPV6 and HPV11 DNA were preponderance in laryngeal benign lesions,and HPV16 DNA were preponderance in laryngeal carcinoma.The relationship between the infection of HPV types and pharyngeal,laryngeal diseases were discussed in this paper.
出处
《耳鼻咽喉（头颈外科）》
2000年第4期238-241,共4页
Chinese Arch Otolaryngology-Head Neck Surg
基金
温州市科技发展计划基金资助项目!(S990 30 6 A)
