摘要
目的:研究GnRH类似物阿拉瑞林诱导体外培养的人肝癌细胞株SMMC-7721发生凋亡的作用,为GnRH类似物用于肝癌的内分泌治疗提供实验资料。方法:采用MTT法、形态学透射电镜观察和末端脱氧核苷酸标记法观察被阿拉瑞林处理后的SMMC-7721细胞的形态学和生化等指标的变化。结果:MTT法研究结果表明阿拉瑞林在10^(-9)mol/L浓度时即可诱导7721细胞凋亡,并呈量-效效应。透射电镜下可观察到时期凋亡细胞和晚期凋亡细胞以及核染色质浓缩并见凋亡小体。末端脱氧核苷酸转移标记法进一步证实阿拉瑞林可以诱导肝癌细胞凋亡并可见调亡小体;与对照组相比,阿拉瑞林处理后TUNEL凋亡指数显著增加(0.29 ± 0.06 vs0.11±0.03,P<0.05)。结论:GnRH类似物可诱导体外培养的肝癌细胞株SMMC-7721发生凋亡,从而提示GnRH类似物对人肝细胞性肝癌具有潜在的治疗作用。
AIM: To induce apoptosis of human cultured hepatocellular carcinoma (HCC) cell line SMMC-7721 by GnRH-analog alarelin, and to provide the experimental evidence for GnRH-A in endocrinotherapy of HCC. METHODS: MTT assay, transmission electron microscopy and DNA end labeling method were used to identify apoptosis of cultured human hepatocellular carcinoma cells treated by alarelin. RESULTS: SMMC-7721 cell line was induced by alarelin in 10^(-9)mol/L concentration. The induction of apoptosis was dose-effect dependent. Under electron microscopy we could identify the earlier and later stage of apoptotic cells, and chromatin condensation, as well as apoptosis body formation. DNA end labeling method showed that alarelin could induce apoptosis of HCC cells (0.29±0.06 vs 0.11±0.03), and their apoptosis body formations were observed. Compared with control group, the TUNEL index was increased significantly in alarelin treated groups (P<0.05). CONCLUSION: GnRH-analog alarelin can induce apoptosis of cultured human HCC cell line SMMC-7721, and it plays a potential role in the treatment of human HCC.
出处
《世界华人消化杂志》
CAS
2003年第9期1329-1332,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金
No.39900142
