摘要
目的:观察TRAIL诱导肝癌SMMC—7721细胞凋亡的作用.方法:采用MTT法检测细胞存活分数;TUNEL法检测细胞凋亡率;流式细胞仪检测细胞凋亡和细胞周期;电镜观察凋亡细胞超微结构.结果:TRAIL对SMMC-7721细胞的存活分数和凋亡率的影响呈典型的量效关系,经TRAIL作用后的细胞,流式细胞仪检测呈标准的亚二倍峰,电镜观察发现经TRAIL作用的部分细胞具有凋亡细胞的典型形态特征.结论:TRAIL可诱导SMMC—7721细胞凋亡.
AIM: To observe the role of TRAIL in inducing apoptosis of SMMC-7721 cells. METHODS: The survival fraction of SMMC-7721 cells was measured by MTT assay; Apoptosis rate was determined by TUNEL method and the ultramicrostructure of apoptosic cells induced by TRAIL was observed by electron-microscopy. RESULTS: The role of TRAIL in survival fraction and apoptosis rate demonstrated a good relationship and the typic structure of apoptosic cells was foud in some cells treated by TRAIL. CONCLUSION: TRAIL can induce apoptosis in SMMC-7721 cells.
出处
《世界华人消化杂志》
CAS
2003年第9期1369-1371,共3页
World Chinese Journal of Digestology
基金
全军"十五"科研基金
No.01MA172
