摘要
目的:观察三氧化二砷对体外培养的人肝癌细胞株细胞端粒酶表达和细胞生长抑制的影响及诱导细胞凋亡的作用,探讨三氧化二砷的抗肿瘤作用及机制.方法:采用噻唑蓝比色分析法(MTT法),流式细胞分析,原位杂交方法以及TUNEL原位末端标记法观察和检测了不同浓度的三氧化二砷对人肝癌细胞株(SMMC-7721)细胞的增生,细胞DNA含量的分布,细胞端粒酶表达的影响及细胞凋亡的诱导作用.结果:20,5,5μmol/L三氧化二砷在24,48,72h时抑制率分别为22.0%,25.7%,32.0%,均能明显抑制人肝癌细胞株细胞的增生(单侧置信区间为10.1,23.7,18.0,P<0.05).流式细胞分析显示加药组G0/G1期细胞比例减少,S期细胞所占比例增高.随着药物浓度的增加细胞端粒酶的表达下降,而凋亡细胞数增加.结论:三氧化二砷能抑制人肝癌细胞增生,其抑制作用具有时间-计量效应关系,并能抑制肝癌细胞端粒酶的表达及诱导肝癌细胞凋亡.
AIM:To investigate the effect of arsenic trioxide on expression of telomerase and induction of apoptosis of hepatoma cells in vitro. METHODS:The human hepatoma cells were incubated with variety concentration of arsenic trioxide. The cell growth and proliferation were detected by MTT assay. DNA distribution in cell was analyzed with flow cytometry. The expression of telomerase was studied by in situ hybridization, and induction of apoptosis was observed with terminal deoxynucleotidy transferase nick and labeling (TUNEL). RESULTS:Inhibitory rate of proliferation of hepatoma cell with arsenic trioxide at 24, 48, 72 hours was 22.0 %, 25.7 %, 32.0 %, respectively (P <0.05). Flow cytometry study indicated that ratio of G0/G1 phase cell was decreased and the portion of S phase cell increased following treatment with arsenic trioxide. The expression of telomerase was decreased and the number of apoptotic cells increased with the increment of arsenic trioxide dosage. CONCLUSION:Arsenic trioxide may inhibit the proliferation of hepatoma cells in a time-and concentration-dependent manner, and suppress the expression of telomerase and induce apoptosis of human hepatoma cells.
出处
《世界华人消化杂志》
CAS
2003年第3期264-267,共4页
World Chinese Journal of Digestology
基金
福建省卫生厅科学研究课题基金资助
No.96038~~
