摘要
目的:探讨CYP2E1 PstI多态与GSTT1空白基因型与胃腺癌的关联。方法:胃腺癌患者56例及健康人对照56名,分别采用PCR-RFLP技术与多重PCR方法,检测CYP2E1基因型和GSTT1空白基因型。结果:CYP2E1 C_1/C_1基因型在胃癌组和健康人对照组中的分布频率分别是69.6％和46.4％,差异具有显著性(x^2=6.27;P<0.05);GSTT1空白基因型在胃癌和健康人中的分布频率分别为 60.7％和 46.4％,在胃癌中分布频率较高,但未有统计学意义(x^2=2.30;0.1<P<0.25);CYP2El基因与GSTT1基因联合分析,结果显示GSTT1空白基因型/CYP2E1(C_1/C_1)基因联合型在胃癌中的分布频率显著高于其他基因联合型,差异具有统计学意义(x^2=3.98;0.025<P<0.05)。结论:CYP2E1 C_1/C_1基因型与胃癌遗传易感性相关;GSTT1空白基因型不增加胃癌的危险性;基因之间具有联合作用,GSTT1非空白基因型/CYP2El(C_1/C_2或C_2/C_2)基因联合是胃癌的保护因素,GSTT1空白基因型/CYP2E1(C_1/C_1)基因联合是胃癌的危险因素,即同时具有GSTT1空白基因型及CYP2E1 C_1/C_1基因型的个体罹患胃癌的危险性显著增加。
AIM: To investigate the relationship between genetic poly- morphisms of CYP4502E1 and GSTT1 and gastric cancer. METHODS: Fifty six patients with histologically confirmed gastric adenocarcinoma (GC case group) and 56 healthy persons (control group), matched by age, sex, smoking, dietary habits and family history of cancer were studied. Genomic DNA samples were assayed for restriction frag- ment length polymorphisms in the CYP2E1 by PCR amplifi- cation followed by digestion with endonuclease PstI, and GSTT1 genes were detected by multiplex PCR. RESULTS: The frequency of CYP2E1 C_1/C_1 genotype was 69.6 % in GC group and 46.4 % in control group, with a statistically significant difference (x^2=6.27; P<0.05, OR=1.915, 95 % CI=1.051-3.489). The frequency of GSTT1 null genotype was higher in GC group (60.7%) than in control group (46.4 %), but the difference was not statis- tically significant (x^2=2.30: 0.1<P<0.25,P=1.783,95 % CI=0.842-3.777). Furthermore, a joint effect of the CYP2E1 and GSTT1 genotypes on cancer risk was observed. GSTT1 non-null genotype/C_1/C_2 or C_2/C_2, genotype was lower than that in GC group (x^2=6.23; 0.01<P<0.025, P=0.302,95 % CI=0.114-0.796). The GSTT1 null genotype/C_1/C_2 genotype in GC group was significantly higher than that in the other groups (x^2=3.98; P<0.05, P=2.250, 95 % CI=1.007-5.026). CONCLUSION. CYP2E1 C_1/C_1 genotype is associated with gastric cancer and individuals who carrry the C_1 allele have a higher risk of developing GC than those with the C_1/C_2 or C_2/C_2 genotype. GSTT1 null genotype does not increase the risk of GC. Combined analysis of polymorphisms has shown that GSTT1 non-null genotype/C_1/C_2 or C_2/C_2 genotype is protective factor of GC ar1d GSTT1 null genotype/C_1/C_2 genotype is a risk factor of GC. That is, individuals with both GSTT1 null genotype and CYP2E1 C_1/C_1 genotype have a greater risk of GC.
出处
《世界华人消化杂志》
CAS
2003年第9期1314-1317,共4页
World Chinese Journal of Digestology
基金
湖北省卫生科技基金
No.WJ01572
