摘要
目的 探讨结直肠癌中脆性组氨酸三联体 (FHIT)基因蛋白表达状况及其与临床病理指标的可能关系。方法 采用兔抗人FHIT蛋白抗体和枸橼酸 微波 SP免疫组织化学方法检测 6 0例甲醛固定、石蜡包埋的结直肠癌蜡块标本中FHIT表达状况并分析其与组织学分级、Dukes分期以及 5年生存率的关系。结果 癌组织FHIT表达强度较正常黏膜高者为 2 1例 (35 0 % ) ,较正常黏膜低者为33例 (5 5 0 % ) ,与正常黏膜基本相等者为 6例 (10 0 % )。FHIT蛋白低表达癌在癌组织学分级中的分布为Ⅰ级癌 7/ 16 ,Ⅱ级癌 14/ 30 ,Ⅲ级癌 12 / 14,各级癌组间比较 ,差异有显著性 (P <0 0 5 )。FHIT蛋白低表达癌在Dukes分期中的分布为A期癌 5 / 11,B期癌 12 / 2 8,C期癌 16 / 2 1,已伴淋巴结转移组的C期癌与未转移组的A、B期癌比较 ,差异有显著性 (P <0 0 5 )。 39例获访病例中 ,FHIT蛋白低表达癌在 5年随访病例组中的分布为 5年生存组为 13/ 2 5 ,5年死亡组为 12 / 14,组间比较差异有显著性 (P <0 0 5 )。结论 结直肠癌FHIT表达状况可能与组织学分级、Dukes分期以及 5年生存率相关 ,提示FHIT表达降低可能对结直肠癌的演化和进展具有一定重要作用 ,并可能成为一个新的预后指标。
Objective To investigate the expression of fragile histidine triad (FHIT) gene protein, FHIT and the possible relationship between FHIT expression and clinicopathological indices in colorectal carcinoma. Methods Detecting FHIT protein expression in 60 cases of formalin-fixed, paraffin-embedded colorectal carcinoma by citrate-microwave-SP immunohistochemical method, and analyzing its relationship to histological grade, Dukes′ stage and 5-year survival rate. Results WT5'BZ]55% of the carcinomas showed a marked loss or absence of FHIT expression compared with their matched normal mucosas. Carcinomas with reduced expression of FHIT correlated with their histological grade, Dukes′ stage ( P <0.05) and 5-year survival rate. The distribution of decreased expression of FHIT was 7/16 in grade Ⅰ carcinoma, 14/30 in grade Ⅱ, 12/14 in grade Ⅲ, respectively. The correlation between decreased expression of FHIT and Dukes′ staging was 5/11 in stage A, 12/28 in stage B, and 16/21 in stage C. The difference between stage A, B with no lymph nodes metastases and the stage C with lymph nodes metastases was of significance ( P <0.05). The follow-up data of 39 cases showed that in the 5-year survival group, 13/25 were of the low FHIT expression carcinomas, while in 5-year deceased group 12/14 were of the low FHIT expression carcinomas ( P <0.05). 55% of the carcinomas showed a marked loss or absence of FHIT expression compared with their matched normal mucosas. Carcinomas with reduced expression of FHIT correlated with their histological grade, Dukes′ stage ( P <0.05) and 5-year survival rate. The distribution of decreased expression of FHIT was 7/16 in grade Ⅰ carcinoma, 14/30 in grade Ⅱ, 12/14 in grade Ⅲ, respectively. The correlation between decreased expression of FHIT and Dukes′ staging was 5/11 in stage A, 12/28 in stage B, and 16/21 in stage C. The difference between stage A, B with no lymph nodes metastases and the stage C with lymph nodes metastases was of significance ( P <0.05). The follow-up data of 39 cases showed that in the 5-year survival group, 13/25 were of the low FHIT expression carcinomas, while in 5-year deceased group 12/14 were of the low FHIT expression carcinomas ( P <0.05). Conclusions The reduced expression of FHIT may be associated with decreasing differentiation, metastasis and 5-year survival rate in colorectal carcinoma. It is suggested that decreased FHIT expression plays an important role in the development and progression of the tumor, and thus may become a new prognostic marker in colorectal carcinoma.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2002年第2期124-127,共4页
Chinese Journal of Pathology
