摘要
目的:探讨GSTM1,GSTT1基因多态与胃腺癌(GAC)临床特征及其与幽门螺杆菌(Hp感染的相关性。方法:应用多重聚合酶链反应(PCR)技术,检测GSTM1,GSTT1基因多态性;^(14)C尿素呼气试验和外周血ELISA法检测Hp感染情况。结果:GAC患者GSTM1空白基因型频率明显高于健康人,其差异有统计学意义(x^2=5.40,P<0.05),GSTT1空白基因型频率在GAC 患者与对照组间的差异无统计学意义;GSTM1空白基因型频率在早期GAC患者与对照组间的差异有统计学意义(x^2=4.74,P<0.05),在进展期、肠型及弥漫型GAC与对照组间的差异均无统计学意义;GSTT1空白基因型频率在肠型GAC患者与对照组间的差异有统计学意义(x^2=4.09,P<0.05),在早期,进展期及弥漫型GAC与对照组间的差异均无统计学意义。GSTM1,GSTT1均为空白基因型的个体患 GAC的危险性是 GSTM1,GSTT1均为非空白基因型个体的 3.38倍(校正 P=3.38,95％CI=1.58-7.51)。GSTM1空白基因型频率,在Hp感染的GAC患者与Hp感染的对照组间的差异有统计学意义(x^2=6.68,P<0.01)。结论:GSTM1空白基因型与GAC易感性有关,肿瘤多处于早期,GSTT1空白基因型与肠型GAC易感性有关,与肿瘤的临床分期无关;GSTM1,GSTT1均为空白基因型的个体患GAC的危险性增加;GSTM1基因多态和Hp感染有协同促GAC发生的作用。
AIM: To study the association of genetic polympphisms of glutathione S-transferase M1 and T1 (GSTM1, GSTT1)with clinicopathological features of gastric adenocarcinoma (GAC) and Helicobacter pylPi (Hp) infection. METHODS: All subjects were unrelated Han people in Hubei Province of China. Using multiplex PCR, we studied the genetic polympphisms of the GSTM1, GSTT1 genes. Hp infection was determined by IgG antibodies to Hp in stped serum samples using enzyme-linked immunosobant assay and ^(14)C urea breath test. RESULTS: The null genotype fP GSTM1 was mPe signifi- cantly common in GACs when compared with controls (x^2=5.40, P<0.05), and mPe common in early stage of GACs when compared with controls too (x^2=4.74, P<0.05). All the differences of the frequency of GSTM1 null genotype between advanced stage GACs P intestinal type carcinomas P diffuse type carcinomas and controls did not reach statistical significance.The null genotype fP GSTT1 was significantly mPe common among intestinat type GACs when compared with controls (x^2=4.09, P<0.05), but all the differences of the frequency of GSTT1 null genotype between early stage GACs P advanced stage GACs P diffuse type carcinomas and controls did not reach statistical significance. The subjects carrying both of the null genotypes fP GSTM1 and GSTT1 had mPe than 3.38-fold risk fP developing GAC compared with the subjects carrying both of the nonull genotypes fP GSTM1 and GSTT1 (adjusted odds ratio, P=3.38,95 % con- fidence interval, CI=1.58-7.51). The null genotype fP GSTM1 was mPe significantly common among those pa- tients with Hp positive GAC compared with Hp positive controls (x^2=6.68, P<0.01). CONCLUSION: The null genotype fP GSTN1 has an in- creased risk of GAC, and most tumPs are in early stage GACs. The null genotype fP GSTT1 is significantly related to the intestinal type GAC,but not significantly related to the tumP stage. Subjects carrying both of the null genotypes fP GSTM1 and GSTT1 have increased risks fP GAC, GSTM1 gene polymPphisms and Hp infection may interact with each other in the initialization of GAC.
出处
《世界华人消化杂志》
CAS
2003年第9期1306-1309,共4页
World Chinese Journal of Digestology
