摘要
目的:探讨胃癌细胞bcl-2基因表达水平与肿瘤细胞增生活性及细胞凋亡程度的关系. 方法:胃癌组织53例,用原位杂交及免疫组化染色法分别检测bcl-2 mRNA,bcl-2蛋白和增生细胞核抗原(PCNA) 的表达,并采用凋亡细胞原位检测方法对组织切片中的凋亡细胞进行观察和比较. 结果:胃癌组织表达bcl-2 mRNA 41例(77.4%),表达Bcl-2 蛋白43例(81.1%),X2检验表明两种方法检测阳性率差异无显著性.胃癌53例增生期细胞标记物PCNA表达及凋亡细胞的阳性率均为100%,细胞凋亡和细胞增生指数呈显著性负相关(r=-0.993,P<0.01).随胃癌细胞Bcl-2蛋白表达水平升高,PCNA阳性细胞指数相应增加,肿瘤凋亡细胞指数则相应减少,Bcl-2蛋白+++组与++组间(t=2.552, 2.699,P<0.05)及前两组分别与-组和+组间(t=4.487, 3.975,2.807,3.094,4.885,5.816,3.404,3.895, P<0.01)凋亡和增生细胞指数差异均有显著性. 结论:胃癌细胞bcl-2基因高表达可引起细胞凋亡减少与过度增生.
AIM: To investigate the relationship between bcl-2 gene expression levels in human gastric carcinoma and the frequency of tumor cell proliferation activity and apoptosis. METHODS: In situ hybridization and immunohistochemis try methods were used to study the frequencies of expressions of bcl-2 gene and nuclear antigen of proliferating cells (PCNA) in 53 gastric carcinomas. Meanwhile, an in situ apoptotic cell detection (TUNEL method) was adopted to compare the number of apoptotic cells and PCNA with the Bcl -2 protein expression in each case. RESULTS: Of the 53 gastric carcinomas, 41 and 43 expressed bcl-2 mRNA and Bcl -2 protein, which were count for 77.4% and 81.1% respectively. There was no significant difference between the positive rates obtained by these two methods. The apoptotic index of gastric carcinomas negatively related to PCNA index (P=-0.993, P<0.01). With the increase of Bcl-2 protein expression , the cell proliferating activity increased but the apoptosis decreased in the tumor cells. Significant difference of cell proliferation and apoptosis existed between +++ group and ++ group of Bcl -2 protein expression (t =2.552, 2.699, P<0.05) as well as between the former two groups and the -, + group (t =4.487, 3.975, 2.807, 3.094, 4.885, 5.816, 3.404, 3.895, P<0.01) respectively. CONCLUSION: The overexpression of bcl-2 gene inhibits cell apoptosis and excessive cell proliferation in the development and progress of gastric carcinomas.
出处
《世界华人消化杂志》
CAS
2004年第11期2543-2546,共4页
World Chinese Journal of Digestology
基金
军队医药卫生"九五"重点课题资助项目
No.96Z047重庆市应用基础研究基金资助项目
No.1998-06~~
