摘要
AIM: To investigate the effect of c9, t1l-conjugated linoleic acid (c9, t11-CLA) on the invasion of human gastric carcinoma cell line and its possible mechanism of preventing metastasis.METHODS: Using reconstituted basement membrane invasion, chemotaxis, adhesion, PAGE substrate zymography and RT-PCR assays, we analyzed the abilities of invasion,direct migration, adhesion of intracellular matrix, as well as the activity of type IV collagenase and expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 mRNA in SGC-7901 cells which were treated with gradually increased concentrations (25, 50, 100 and 200μmol/L) of c9,c11-CLA for 24 h.RESULTS: At the concentrations of 200μmol/L, 100μmol/L and 50μmol/L, c9,tll-CLA suppressed the invasion of SGC-7901 cells into the reconstituted basement membrane by 53.7 %, 40.9 % and 29.3 %, respectively, in comparison with the negative control. Only in the 200 μmol/L c9,tll-CLA group, the chemotaxis of SGC-7901 cells was inhibited by 16.0 % in comparision with the negative control. C9,tll-CLA also could inhibit the adhesion of SGC-7901 cells to laminin, fibronectin and Matrigel, increase the expression of TIMP-1 and TIMP-2 mRNA, and reduce type IV collegenase activities in the serum-free medium supernatant of SGC-7901 cells.CONCLUSION: c9,t11-C:LA can inhibit the invasion of SGC-7901 cells at multiple procedures in tumor metastasis cascade, which may be associated with the induction of TIMP-1 and TIMP-2 mRNA expression.
AIM:To investigate the effect of c9,t11-conjugated linoleic acid(c9,t11-CLA)on the invasion of human gastric carcinoma cell line and its possible mechanism of preventing metastasis. METHODS:Using reconstituted basement membrane invasion,chemotaxis,adhesion,PAGE substrate zymography and RT-PCR assays,we analyzed the abilities of invasion, direct migration,adhesion of intracellular matrix,as well as the activity of type Ⅳ collagenase and expression of tissue inhibitor of metalloproteinase(TIMP)-1 and TIMP-2 mRNA in SGC-7901 cells which were treated with gradually increased concentrations(25,50,100 and 200 μmol/L)of c9,t11-CLA for 24 h. RESULTS:At the concentrations of 200 μmol/L,100 μmol/L and 50 μmol/L,c9,t11-CLA suppressed the invasion of SGC- 7901 cells into the reconstituted basement membrane by 53.7 %,40.9 % and 29.3 %,respectively,in comparison with the negative control.Only in the 200 μmol/L c9,t11- CLA group,the chemotaxis of SGC-7901 cells was inhibited by 16.0 % in comparision with the negative control.C9,t11- CLA also could inhibit the adhesion of SGC-7901 cells to laminin,fibronectin and Matrigel,increase the expression of TIMP-1 and TIMP-2 mRNA,and reduce type Ⅳ collagenase activities in the serum-free medium supernatant of SGC- 7901 cells. CONCLUSION:c9,t11-CLA can inhibit the invasion of SGC- 7901 cells at multiple procedures in tumor metastasis cascade,which may be associated with the induction of TIMP-1 and TIMP-2 mRNA expression.
基金
the National Natural Science Foundation of China, No.30070658
