摘要
目的:通过检测E-钙黏素(E-Cad)、MMP2和ICAM-1在胃癌不同证型的表达,探讨基因表达与胃癌中医证型的关系.方法:胃癌标本59例,其中肝胃不和型10例、脾胃虚寒型10例、胃热伤阴型9例、痰湿凝结型10例、瘀毒内阻型10例和气血双亏型10例;采用逆转录-聚合酶链反应(RT-PCR)检测六型胃癌组织E-Cad、MMP2和ICAM-1mRNA的表达水平,并对各基因在不同证型的表达情况以及证型与基因的关系进行分析.结果:3种目的基因在6种证型中的表达均有很大差异,其中,E-Cad在胃热伤阴型与气血双亏型中的表达有显著性差异(0.58±0.11vs0.89±0.25,P=0.026),MMP2在肝胃不和型与瘀毒内阻、气血双亏两型有显著性差异(0.78±0.26vs1.27±0.44,P=0.006;0.78±0.26vs1.30±0.59,P=0.004),胃热伤阴型与瘀毒内阻、气血双亏两型有显著性差异(0.86±0.22vs1.27±0.44,P=0.024;0.86±0.22vs1.30±0.59,P=0.016),脾胃虚寒型与瘀毒内阻、气血双亏两型有显著性差异(0.86±0.22vs1.27±0.44,P=0.020;0.86±0.22vs1.30±0.59,P=0.013);ICAM-1在各证型间无显著差别,但已有组间差别的趋势.结论:不同证型胃癌的转移机制存在一定的差异,基因表达的差异可能是形成不同证型的物质基础之一.
AIM： To investigate the expression of E-cadherin （E-Cad）, matrix metalloproteinase-2 （MMP-2） and intercellular adhesion molecule-1 （ICAM-1） in different traditional Chinese medicine （TCM） classifications of syndrome for gastric carcinoma, and explore their relationships with syndromic classifications of gastric cancer.
METHODS： A total of 59 gastric cancer cases, including the types of incoordination between liver and stomach （n = 10）, insufficiency of spleen and stomach （n = 10）, stomach heat due to yin deficiency （n = 9）, stagnation of phlegmdamp （n = 10）, retention of blood stasis toxin in the interior （n = 10） and exhaustion of both qi and blood （n = 10）. The expression of E-Cad, MMP2, and ICAM-1 mRNA was determined by reverse transcription-polymerase chain reaction （RT-PCR）, and the diversities were analyzed between the different syndromic types.
RESULTS： Evident diversity was noted in the different syndromic types. The expression of E-Cad was significantly different between the types of stomach heat due to yin deficiency and exhaustion of both qi and blood （0.58 ± 0.11 vs 0.89 ± 0.25, P = 0.026）. The level of MMP2 expression was markedly lower in the types of incoordination between liver and stomach, stomach heat due to yin deficiency, and insufficiency of spleen and stomach than that in the type of retention of blood stasis toxin in the interior （0.78 ± 0.26, 0.86 ± 0.22, 0.86 ± 0.22 vs 1.27 ± 0.44; P= 0.006, P = 0.024, P = 0.020） or exhaustion of both qi and blood （0.78 ± 0.26, 0.86 ± 0.22, 0.86 ±0.22 vs 1.30 ± 0.59, P = 0.004, P = 0.016, P = 0.013）. The expression of ICAM-1 was not evidently diversified between the six syndromic types.
CONCLUSION： The metastasic mechanism of gastric cancer is diversified between the different syndromic classifications, in which the differences of gene expression may play important roles.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第36期3461-3465,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30271626~~
关键词
E-钙黏素
基质金属蛋白酶-2
细胞间黏附分子-1
中医证型
胃癌
E-cadherin, Matrix metalloproteinase 2
Intercellular adhesion molecule-Z
Traditional Chinese Medicine types of syndrome, Gastric carcinoma
