摘要
目的 采用HPLC法改进的流动相系统测定氧氟沙星胶囊中氧氟沙星的含量。方法 色谱柱 :TechsphereC1 8ODS柱(1 5 0mm× 4 6mm ,5 μm) ,流动相 :0 0 5mol·L-1 枸橼酸 -乙腈 -四乙胺 (70∶2 8∶2 ) ,流速 :0 8ml·min-1 ,UV检测波长 :2 93nm。结果 氧氟沙星在 2 0 4～ 1 0 2 0mg·L-1 范围内 ,浓度与峰面积线性关系良好 (r =0 9999) ,日内RSD =1 0 7% (n =5 ) ,日间RSD =1 2 3% (n =3)。平均回收率为 99 98% (n =5 )。结论 改进后方法简便、快速、结果准确 。
AIM To improve the HPLC method for determination of Ofloxacin capsules. METHODS A Techsphere C18 column (150 mm×4 6 mm,5 μm) was used. A mobil phase consisted of citric acid (0 05 mol·L -1 ) acetonitrile tetramethylethylenediamine(70∶28∶2)was adopted. The flow rate was 0 8 ml·min -1 . The detection wavelength was 293 nm. RESULTS There was a good linear correlation in the range of 20 4～102 0 mg·L -1 ( r =0 9999), within day RSD=1 07%( n =5), between day RSD=1 23%( n =3). The average recovery was 99 98%( n =5). CONCLUSION The improved methods is simple,rapid and accurate,and is suitable for the quality control of Ofloxacin capsules.
出处
《安徽医药》
CAS
2003年第5期372-373,共2页
Anhui Medical and Pharmaceutical Journal
