摘要
目的 :建立实验性小鼠前胃癌肝转移模型。方法 :将浓度为 1× 10 7个·ml-1的小鼠前胃鳞癌细胞 (MFC)悬液 0 2ml接种于 615小鼠脾被膜下 ,分别于术后第 1、2、3、4周处死 2～ 3只小鼠 ,观察腹腔内肿瘤生长情况 ,其余小鼠待其自然死亡 ,观察生存天数。结果 :肝转移发生率为 83 3 3 % ,小鼠平均荷瘤生存时间为 (3 7 83± 7 3 2 )d。病理结果提示肝转移瘤细胞与脾脏肿瘤细胞形态学相似 ,符合低分化鳞癌的特征。结论 :本研究成功建立了实验性小鼠前胃癌肝转移模型 。
Objective To establish a preventriculus squamous carcinoma liver metastases model for experimental studies in mice.Methods Liver metastases were established in 615 mice by intrasplenic injection of preventriculus squamous carcinoma cells(MFC) in a concentration of 2×10 6 cells in 0 2 ml solution.After surgery,2～3 mice were sacrificed each week,and the others were used to observe the survival time.The liver metastases were investigated pathologically in all animals.Results The incidence of liver metastases was 83 33%,the average survival time of tumor bearing animals was (37 83±7 32) days.The pathological results showed that tumor cells of liver metastases were morphologically similar with spleen tumor cells in a accordance with poorly differentiated preventriculus squamous carcinoma.Conclusion This study successfully established the model of preventriculus squamous carcinoma liver metastases in mice.It's a useful model to select antineoplastics and to study metastatic behaviors.
出处
《东南大学学报（医学版）》
CAS
2003年第5期313-315,共3页
Journal of Southeast University(Medical Science Edition)
