摘要
目的通过盲肠造疝原位接种瘤块法建立小鼠大肠癌肝转移模型。方法将CT26细胞接种到BALB/c小鼠项背部,约10d后获得实体瘤,再对30只BALB/c小鼠实行盲肠造疝原位接种瘤块术,术后随机将小鼠分为A,B两组。A组20只,分别于术后1,2,3,4周随机抽取5只用颈椎脱臼法处死,剖腹观察原位移植瘤体积大小、肝转移癌情况、其他脏器受累情况及有无腹水等,并对肝组织进行连续病理切片,HE染色,以观察肝转移情况。B组10只,观察其自然生存期。结果盲肠造疝原位接种瘤块术后小鼠自然生存时间为(30.4±2.7)d。术后1,2周肝组织病理检查未见转移灶,术后3周1只(1/5)出现肝转移灶,术后4周3只(3/5)出现肝转移灶。结论通过盲肠造疝原位接种瘤块法成功建立了小鼠大肠癌肝转移模型,并且是研究大肠癌肝转移的理想模型。
Objective To establish a model of hepatic metastasis of colorectal cancer in mice by orthotopic transplantation with fresh tumor tissues on herniated cecum. Methods CT26 cells were injected subcutaneously on the nucha of BALB/c mice. Tumor was formed 10 days later. Orthotopic transplantation with fresh tumor tissues on herniated cecum was performed in 30 BALB/c mice, which were romdomly divided into two groups. Group A contained 20 mice. Groups of 5 mice were sacrificed by cervical dislocation on the day 7,14,21 and 28 respectively. The volume of tumor in situ, liver metastasis and involement of other organs were examined. Serial sections were performed to observe liver metastasis focus. Group B contained 10 mice. Survival was recorded. Results The Survival after orthotopic transplantation was （30. 4 ± 2. 7） days. There was no liver metastasis week of 1 and 2. There was liver metastasis in one （ 1/5 ） mouse week 3 and 3 （3/5） week 4. Conclusion A model of hepatic metastasis of colorectal cancer in mice can be created by orthotopic transplantation with fresh tumor tissues on herniated cecum and it is an ideal model for study of hepatic metastasis of colorectal cancer.
出处
《广东医学》
CAS
CSCD
北大核心
2007年第6期855-857,共3页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:30470435)
教育部留学回国人员科研启动基金资助项目(编号:教外司留[2003]406号)
广东省自然科学基金资助项目(编号:031766)
广东省科学技术厅科技计划项目(编号:2004B31201015)
广州市教育局科技计划项目(编号:1025)
关键词
结肠直肠肿瘤
肿瘤转移
小鼠
动物模型
Colorectal neoplasm Neoplasm metastasis Mice Animal model
