摘要
以金银花为原料,经过超高压处理后,加入乙醇进行提取,经浓缩纯化后,得到金银花绿原酸提取物。通过单因素实验,以金银花绿原酸得率为指标,研究了提取压力、保压时间、提取时间、料液比、乙醇浓度对金银花绿原酸得率的影响;在此基础之上,通过响应面优化实验对超高压处理提取金银花绿原酸工艺进行优化。结果表明,超高压处理提取金银花绿原酸的最优条件为压力325 MPa,保压时间10 min,提取时间2 h,料液比1∶10 g/mL,乙醇浓度60%。在此条件下,绿原酸得率为4.872%±0.049%。表明超高压处理能提高金银花绿原酸的产量。
Honeysuckle chlorogenic acid was extracted from honeysuckle by adding ethanol after ultra-high pressure treatment.After concentration and purification,the chlorogenic acid extract of honeysuckle was obtained. The extraction conditions (extraction pressure,holding time,extraction time,liquid/solid ratio and ethanol concentration) were studied according to the combination of single factor experiment and response surface methodology (RSM).The optimal extraction condition was as follows: the pressure was 325 MPa,the holding time was 10 min,the extraction time was 2 h,the liquid/solid ratio was1∶ 10 g/mL,ethanol concentration was 60%,with an extraction rate of 4.872% ± 0.049%. It was showed that ultra-high pressure treatment could increase the yield of honeysuckle chlorogenic acid.
作者
浦娜娜
王成忠
李东荣
PU Na-na;WANG Cheng-zhong;LI Dong-rong(Qilu University of Technology(Shandong Academy of Sciences),Ji’nan 250000,China;Shandong Shangshui Agricultural Development Co.,Ltd.,Zibo 255000,China)
出处
《食品工业科技》
CAS
北大核心
2019年第5期201-206,共6页
Science and Technology of Food Industry
关键词
金银花
超高压
绿原酸
得率
响应面法
honeysuckle
extra-high pressure
chlorogenic acid
yield
response surface methodology
