摘要
BRD7基因是与鼻咽癌相关的候选抑瘤基因 .为了进一步研究该基因的作用机制 ,将pcDNA3 1(+) /BRD7的表达质粒经脂质体导入HNE1细胞 ,RNA斑点杂交筛选出阳性克隆 ,通过双向电泳分离过表达BRD7的HNE1细胞内蛋白质 ,筛选出差异表达的蛋白质点 ,并进行质谱分析鉴定 .鉴定出 10种表达上调的蛋白质点 ,包括精氨 (基 )琥珀酸裂解酶 ,TSA (thio specificantioxdant) ,Proteaseomeactivator2 8betasubunit (PA2 8) ,金属蛋白酶抑制因子 2前体等 ,这些蛋白质涉及细胞生长 ,细胞代谢等很多相关事件 .
BRD7 gene is a novel candidate tumor suppression gene associated with NPC. In order to study the function of BRD7, BRD7 was introduced into HNE1 cells by liposome transfection. After staining and image analysis, the ten differential expression spots which up-regulated in BRD7 transfected cells were isolated and identified by two-dimensional polyacrylamide gel eletrophoresis (2D PAGE) and MALDI-TOF. These proteins included argininosuccinate lyase, TSA, proteaseome activator 28 beta subunit, metalloproteinase inhibitor-2 precursor, which involved in cell cycling, transcription regulation, metabolism and so on. The results indicated that the BRD7 gene may play effect on NPC cells by up-regulating the expression of these proteins.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2003年第5期721-725,共5页
Progress In Biochemistry and Biophysics
基金
国家重点基础研究发展规划项目 ( 973 ) (G19980 5 10 0 8)
国家自然科学基金资助项目 ( 3 0 0 0 0 0 65 )
国家高技术"863"计划资助项目 ( 10 2 10 0 1 0 5 )~~
关键词
鼻咽癌
蛋白质表达谱
BRD7基因
抑瘤基因
BRD7 gene
cell transfection
northern spot
two-dimensional gel eletrophoresis
MALDI-TOF
