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鼻咽癌血清蛋白质双向电泳图谱分析

Analysis of Two-Dimensional Gel Electrophoresis Map of Serum Proteins in Patients with Nasopharyngeal Carcinoma
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摘要 目的:探讨和分析鼻咽癌(NPC)差异表达的血清蛋白质。方法:以NPC和正常血清标本为研究对象,利用双向电泳(2-DE)分离NPC和正常血清蛋白质,银染显色,Umax扫描仪获取图像,Melanie4软件分析图像,寻找鼻咽癌相关差异蛋白质。结果:建立了较稳定的NPC和正常血清蛋白质2-DE图谱,平均蛋白质点数约400个。软件分析显示2组共有11个差异蛋白质点,10个仅在NPC血清中出现,1个仅在正常血清中出现。结论:差异蛋白质的分离为NPC的相关血清蛋白质的鉴定和其他肿瘤血清蛋白质组学研究奠定了基础。 Objective：To detect and analyze the differentially expressed serum proteins in patients with nasopharyngeal carcinoma（NPC）.Methods：Two-dimensional gel electrophoresis（2-DE） were used to study and separate proteins in sera of NPC patients and normal objects.The gels were stained by sliver,scanned by Umax canner,analyzed by Melanie 4 software to find differential protein spots associated with NPC.Results：Two-dimensional gel electrophoresis maps from sera of NPC pa-tients and normal objects were constantly obtained.The average protein spots were 400.The map analysis by software revealed 11 differential protein spots between two groups.Ten differential protein spots were identified specifically in NPC serum and 1 protein spot in normal serum.Conclusion：The separation of differentially expressed proteins from NPC serum would be use-ful for further identifying proteins associated with NPC and for other related serum proteomic in tumor research.
作者 李雪华 李欣 徐守军 赵亮 丁彦青
机构地区 武警医学院病理学教研室、天津市职业与环境危害生物标志物重点实验室 南方医科大学 解放军军事医学科学院
出处 《天津医药》 CAS 北大核心 2008年第11期833-835,共3页 Tianjin Medical Journal
基金 国家重点基础研究发展计划973项目(项目编号:2001CB510208) 天津市科委自然科学基金面上项目(项目编号:06YFJMJC09000) 武警医学院科研基金(项目编号:WY2006-8)
关键词 鼻咽肿瘤 电泳 凝胶 双向 癌 血清 蛋白质组学 nasopharyngeal neoplasms electrophoresis,gel,two-dimensional carcinoma serum proteomics
分类号 R446.112 [医药卫生—诊断学] R383.24 [医药卫生—医学寄生虫学]
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参考文献10

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二级参考文献21

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天津医药
2008年 第11期

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