摘要
目的获得高凝状态大鼠主动脉上调表达新基因HCR2的全长cDNA,并对该基因进行生物信息学分析。方法从高凝大鼠主动脉中表达显著上调的序列标签(EST,GenBank登录号为BQ901227)出发,利用cD-NA末端快速扩增(rapidamplificationofcDNAend,RACE)和巢式PCR技术扩增其全长cDNA,利用NCBI的数据库对其进行生物信息学分析。结果成功地获得了HCR2的全长cDNA,在GenBank中的登录号为AY234417。用RT-PCR证实HCR2是高凝大鼠表达上调基因。生物信息学分析显示,该基因cDNA序列全长为1275bp,定位于大鼠染色体4q11,编码由78个氨基酸组成、相对分子质量为8841.7、pI为8.59的蛋白质;无信号肽序列和跨膜序列,很可能是一种核蛋白;与已知蛋白无明显同源性。结论HCR2的成功克隆为进一步研究其生物学功能及其在高血凝的发生、发展中的作用奠定了坚实基础。
Objective The aim of this study was to clone the full-length cDNA of HCR2 up-regulated in aorta of hypercoagulable rat and make the relevant bioinformatic analysis. Methods Rapid amplification of cDNA end （RACE） and nested PCR technique was used to amplify the full-length cDNA of HCR2 from an EST（GenBank accession number BQ901227）which was significantly up-regulated in hypercoagulable rat. Results The full-length cDNA of HCR2 has been obtained （GenBank accession number AY234417）. Semi-quantitative RT-PCR demonstrated that HCR2 is up-regulated in aorta of hypercoagulable rat. Bioinformatic analysis showed that HCR2 gene is located in rat chromosome 4q11. The full-length cDNA of HCR2 is 1275 bp, coding for a 78 amino acids polypeptide with a theoretical molecular weight of 8841.7 and isoelectric point of 8. 59. This protein has no signal peptide and transmembrane sequence. It may be a nuclear protein. It shares no significant homology with any known protein. Conclusion The successful cloning of HCR2 has laid a solid foundation for further study of its function and its possible role in the occurrence and development of hypercoagulability.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期601-604,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家973计划(项目编号G2000056910)
四川大学校青年基金(0040105505020)资助
