摘要
目的探讨有氧运动对载脂蛋白ApoE基因缺陷小鼠动脉粥样硬化斑块形成、血清一氧化氮(NO)浓度、血清及肝脏超氧化物歧化酶(SOD)活性以及胸主动脉脂质过氧化的影响,以及有氧运动调节血脂代谢以外的抗动脉粥样硬化的可能机制。方法10周游泳运动后,测定动脉粥样硬化斑块面积,血清NO浓度,血清及肝脏SOD活性;应用冷Schiff组织化学法结合计算机模拟图像定量分析法检测胸主动脉的脂质过氧化反应,脂质过氧化阳性反应区呈紫红色。常规HE染色;胸主动脉矢状面油红-O大体染色。结果运动组ApoE基因缺陷小鼠动脉粥样硬化斑块平均面积小于对照组(P<0.01);血清NO浓度(P<0.01)及SOD活性(P<0.05)高于对照组,但肝脏SOD活性与对照组相比没有显著性差异(P=0.056);胸主动脉的脂质过氧化程度显著低于对照组(P<0.01);血管壁及内膜损伤程度相对较轻。结论有氧运动通过增强机体抗氧化能力,提高生物活性形式的NO浓度及SOD活性,改善血管内皮功能,延缓动脉粥样硬化斑块的发生发展,可能属独立于血脂调节作用以外的其他抗动脉粥样硬化机制。
Aim To observe the influence of aerobic training on the formation of at herosclerotic lesions, the concentration of nitric oxide(NO) in serum , the acti vity of superoxide dismute(SOD) in serum and liver and the lipid peroxidation in thoracic aorta in ApoE-deficient mice in order to understand the mechanisms be yond lipid-regulation of aerobic training in anti-atherosclerosis. Methods Six teen ApoE gene deficient mice were equally divided into two groups: swimming gro up and control group. The atherosclerotic lesions,the serumal NO,the SOD activit y of serum and liver were determined. Lipid peroxidation was detected using Cold Schiff histochemical method combined with computer image quantitative analysis technique on frozen sections of thoracic aorta. The response area of positive li pid peroxidation presents purplish red. Harris'hematoxylin(HE) staining was used on frozen sections of thoracic aorta.Oil Red O(ORO) staining was used on the in tima of the thoracic aorta. Results Compared with controls,aerobic training dela yed the plaque formation of atherosclerosis in ApoE-deficient mice(P< 0.01);the concentration of serumal NO was increased(P< 0.01);the activity of SOD in serum was increased(P< 0.05), but the SOD activity of liver showed no significant dif ference between the two groups (P=0.056);the lipid peroxidative reaction of thor acic aorta was weaker(P< 0.01); the injures of vessel dissepiment and endometriu m were slighter.Conclusion These data demonstrate that aerobic training signific antly increases anti-oxidation function,which plays an important role in the im provement of endothelial function, the anti-inflammatory effect and prevention of the onset and development of atherosclerotic plaques in ApoE-deficient mice. It may reflect a new mechanism of aerobic training on the anti-atherosclerosis, independent of lipid-regulation mechanism.
出处
《中国临床康复》
CSCD
2003年第18期2626-2627,共2页
Chinese Journal of Clinical Rehabilitation
基金
江苏省教委课题(99KJD890001)
扬州大学课题(E9909524)~~
