摘要
目的:探讨具有降血糖、调血脂、改善糖耐量、抗氧化等作用的荞麦种子提取物对糖尿病大鼠血浆及肾脏中糖基化终产物形成的影响,以及量效关系效应。方法:实验于2004-03/07在华北煤炭医学院药理教研室完成。选用SD大鼠75只,随机分为5组,每组15只。①正常对照组未造成糖尿病模型,用9g/L的生理盐水80mg/kg腹腔注射。②模型对照组灌胃与正常对照组相同容积的常用水。③需造模的大鼠均禁食16h,腹腔注射链脲佐菌素80mg/kg,72h后取尾静脉血测血糖,凡空腹血糖≥15mmol/L作为糖尿病大鼠,并随机分4组,每组15只。荞麦种子提取物0.1,0.2,0.4g/(kg·d)治疗组分别灌胃荞麦种子提取物0.10,0.20,0.40g/kg。上述各组均每天给药1次,连续12周。末次给药后禁食12h,取尾静脉血测空腹血糖、测定血浆及肾组织匀浆上清果糖胺、糖基化终产物含量。空腹血糖测定采用葡萄糖氧化酶法。果糖胺测定按南京建成生物工程研究所提供的试剂盒要求进行。糖基化终产物含量(荧光强度)测定采用荧光分光光度计完成。计量资料差异性测定采用t检验。结果:纳入大鼠75只,每组15只,实验过程中因部分大鼠造模失败脱失,最终进入结果分析大鼠59只,正常对照组、模型对照组、荞麦种子提取物0.1,0.2,0.4g/(kg·d)治疗组分别为15,10,11,12,11只。①大鼠血糖及血浆和肾组织果糖胺含量:荞麦种子提取物各剂量治疗组均明显低于模型对照组,且剂量越大,所测血及肾组织指标含量越低,呈显著剂量依赖性(P<0.05～0.01);荞麦种子提取物各剂量治疗组高于正常对照组,但无明显差异(P>0.05);模型对照组高于正常对照组(P<0.01)。②血浆糖基化终产物水平:荞麦种子提取物各剂量治疗组均低于模型对照组,随着其剂量增高,水平逐渐下降,但仅在0.40g/kg时差异明显(P<0.05);肾组织糖基化终产物含量:荞麦种子提取物各剂量治疗组均明显低于模型对照组,且剂量越大,含量越低,呈显著剂量依赖性(P<0.01)结论:荞麦种子提取物对糖尿病大鼠血浆、肾组织果糖胺的生成有明显抑制作用,对肾组织糖基化终产物产生的影响也如此,且呈显著量效关系;但对血浆非酶糖基化终产物产生抑制作用仅在高剂量时明显。
AIM: To investigate the influence of extract of buckwheat seed on the advanced glycation end products(AGEs) and plasm in diabetic rats and probe into its dose-depent effect based on the abilites of extract of buckwheat seed such as decreasing blood glucose, regulating blood lipid, improving glucose tolerance and antioxidation. METHODS: The experiment was carried out in the Department of Pharmacology, North China Coal Medical College from March to July 2004. Seventy-five SD rats were randomly divided into 5 groups with 15 in each.①Rats in the normal control group was not induced to diabetes mellitus(DM), and intraabdominally injected with 9 g/L normal saline 80 mg/kg body mass. ②Rats in the model control group was gastrically infused common-used water with the same volume of as the above group. ③The rats were fasting for 16 hours before modeling, and then injected with streptozotocin 80 mg/kg intraabdominally to induce DM. After 72 hours, the level of blood glucose was detected in venous blood of tails. The rats with fasting blood glucose(FBG) equal to or more than 15 mmol/L served as diabetic rats, and then were randomly divided in to 4 groups with 15 in each. Extract of buckwheat seed 0.1, 0.2, 0.4 g/kg was given to the rats of the extract of buckwheat 0.1, 0.2, 0.4 g/(kg·d) groups. All the rats were administrated once a day for 12 consecutive weeks, and at the end of administration the rats were fasting for 12 hours. The venous blood were drawn form the rats' tails to detect the levels of FBG by glucose oxidase, the cortents of AGEs(intensity of fluorescence) in plasma and renal homogenate supernatant by spectrofluorometer. The detection of fructosamine content in plasm and renal homogenate supernatant was performed by using the test kits supported by Nanjing Jiancheng Institute of Biotechnology. Difference of measurement data was analyzed by t test. RESULTS: In the 75 rats used (15 in each group), 59 were analyzed in the result due to failed modeling, and 15, 10, 11, 12, 11 were in the normal control, model control, extract of buckwheat seed 0.1, 0.2, 0.4 g/kg per day groups respectively. ①The levels of FBG and the content of fructosamine in plasm and renal tissues were significantly lower in the extract buckwheat seed groups than in the model control group, and were decreased with the increase of dosage in a dose-dependent manner(P < 0.05 to 0.01); They were higher in the extract buckwheat seed groups than in the normal control group, but insignificantly(P > 0.05), and significantly higher in the model control group than in the normal control group(P < 0.01). ②The level of AGEs in plasm was lower in the extract buckwheat seed groups than in the model control group, and was decreased with the increasing dosage of extract buckwheat seed, but the difference was only significant at the dose of 0.40 g/kg(P < 0.05). The level of AGEs in the renal tissues significantly lower in the extract buckwheat seed groups than in the model control group, and was decreased with the increase of dosage in a dose-dependent manner(P < 0.01). CONCLUSION: Extract of buckwheat seed has remarkably inhibitory effect on the production of fructosamine and AGEs in plasm and kidney of diabetic rats in a dose-dependent relationship; However, its inhibitory effect on non-enzymatic AGEs was obvious only at a high dosage.
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第23期138-139,共2页
Chinese Journal of Clinical Rehabilitation
