摘要
介绍了卡那霉素在转基因植物筛选中的作用机理及其在植物转化过程和转化后代中的应用现状。卡那霉素不仅在植物转化过程中可起到筛选作用 ,而且在转化后代中可通过其对后代进行遗传分析和测定种子纯度 ,同时还可用于后代田间成株的筛选。随着卡那霉素的广泛使用 ,卡那霉素抗性基因的安全性问题日益受到重视。概述了转基因植物的杂草化、卡那霉素抗性基因的水平扩散。
The functional mechanism and applications of Kanamycin in screening transgenic plants and their progenys were reviewed. Kanamycin could apply to distinguish the transformants from non transformants during transformation,and to genetic analyses of transformed progenys,or purity assay of seeds,moreover to screen the mature plants of transformed progenys in the field . With the wide application of Kanamycin,its biosafety was more and more emphasized .In this paper,the advances were summarized on the weeding of transgenic plants ,horizontal expansion of Kanamycin gene, and its safety for medicine and food.\;
出处
《中国生物工程杂志》
CAS
CSCD
2003年第6期9-13,共5页
China Biotechnology
基金
863基金项目资助 (10 1 0 1 0 1 0 1)
