摘要
通过土壤农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍ ｔｕｍｅｆａｃｉｅｎｓ）的介导，将烟草花叶病毒（ＴＭＶ）的５４×１０３蛋白ｃＤＮＡ转入番茄植物中，得到了转基因植株．这些植株抗卡那霉素，具有胭脂碱合成酶的活性．ＤＮＡ的ＰＣＲ扩增及Ｎｏｒｔｈｅｒｎ ｂｌｏｔ分析表明，５４×１０３蛋白基因已整合到番茄基因组上．攻毒实验表明，未转基因的对照组接种病毒２周后即出现花叶，并且随着时间的推移，花叶症状更加典型．而转基因的植物接种病毒后一直到开花结果都未见发病，即没有花叶出现．
The cDNA encoding 54 x 103 protein of tobacco mosaic virus(TMV)is introducedinto tomato by Agrobacterium-mediated method and transgenic plants are obtained.Nopalinesynthtase activity is demonstrated in the kanamycin-resistance lantlet.It is demonstrated thatTMV 54 x 103 protein gene has been integrated into the genomes of tomato plants by PCR andNorthern blot. The transgenic tomatoes show no typical mosaic symptom after TMV infection inthe Whole life while the control plants show typical mosaic synptoms in 2 weeks after TMVinfection.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第1期93-96,共4页
Journal of Beijing Normal University(Natural Science)
基金
国家重点实验室项目
关键词
烟草花叶病毒
蛋白基因
转基因番茄
番茄
抗性
TMV 54x103 protein gene
Agrobacterium tumefaciens
transgenic tomato
resistance
