摘要
采用附加不同浓度卡那霉素(Kan)的1/2MS培养基对转基因油青菜心T1代种子进行培养,快速鉴定转基因菜心T1代种子对Kan的敏感性,结果表明:转基因菜心幼苗的白化率随着Kan浓度的升高而增大,其中以Kan附加浓度为60 mg/L时较适合于菜心转化植株后代的选择鉴定。
The T1 seeds were cultured on 1/2MS substrate adding kanamycin with different concentration to identify the sensitivity of T1 seeds of transgenic Brassica parachinensis to kanamycin rapidly. The Results showed that the albefaction rate of transgenic plantlets increased with the elevation of kanamycin concentration. 60 mg/L kanamycin could select and identify transgenic plantlets offspring effectively.
出处
《广东农业科学》
CAS
CSCD
北大核心
2009年第7期63-64,共2页
Guangdong Agricultural Sciences
基金
国家自然科学基金(30800750)
安徽农业大学引进人才科研资助项目(yj2008-2)
关键词
菜心
卡那霉素
转基因
种子
Brassica parachinensis
kanamycin
transgene
seed
