摘要
以草莓品种宝交早生的花药愈伤组织建立的悬浮细胞系为试材,对原生质体分离和再生进行研究.结果表明:酶液的渗透压、浓度和配比对悬浮细胞原生质体产量和活力有重要的影响.悬浮细胞在CPW+1.0%Cellulase R-10+0.5 Macerozyme R-10+0.05%Pectolyase Y-23+0.6 mol/L甘露醇+0.5%PVP的酶液中酶解12 h,原生质体产量和活力最高,分别达16.35×106/g和84.6%.采用液体浅层培养法对原生质体进行培养获得了再生愈伤组织.
The conditions of establishment of suspension cell lines from anther-derived calli, protoplast isolation and regeneration of strawberry Hokowase were studied. Osmotic pressure, concentration and ratio of enzyme solution affected the yield and viability of protoplasts. The protoplast yield and viability could amount to 16.35×106 protoplasts/gFW and 84.6% respectively when suspension cultures were incubated in enzyme solution containing CPW salts, 1.0%Cellulase R-10, 0.5% Macerozyme R-10,0.05%Pectolyase Y-23 ,0.6 mol/L mannitol and 0.5% polyvinylpyrrolidone for 12 hours. Protoplasts were cultured in thin-layer liquid medium, and protoplast-derived calli were obtained.
出处
《果树学报》
CAS
CSCD
北大核心
2003年第4期319-321,共3页
Journal of Fruit Science
关键词
草莓
原生质体
培养
愈伤组织
Strawberry(Fragria ananassa Duch.)
Protoplast
Culture
Callus
