摘要
从中华猕猴桃（Actinidia chinensis Planch.）子叶愈伤组织系A14N7分离的原生质体,培养在改良NN-69培养基中,7—10天发生第一次细胞分裂,20天的分裂率为10%左右。原生质体最适植板密度是5×104/ml;培养方法以液体浅层培养最好。4个月左右,按分步诱导法,把原生质体来源的小愈伤组织逐步转移到不同的再生培养基上诱导器官分化,最后得到原生质体再生植株。
Protoplasts isolated from cotyledon callus line of A_(14)N_7 of Actinidia Chinensis Planch, were cultured in the improved NN-69 medium.First division of regenerated cells occurred during 7-10 days of culture,and percentage of the cell division was about 10% at day 20. The best result of protoplast culture was achieved when protoplasts were cultured in liquid medium at a density of 5×10~4/ml.About 4 months,protoplast-derived calli were transferred stepwisely onto differentiation media where they developed into green compact calli,from which the perfect plants were regenerated.
关键词
中华猕猴桃
原生质体
再生植株
Actinidia chinensis
Protoplasts
Plant regeneration
