摘要
目的 了解表皮生长因子 (EGF)和胰岛素样生长因子 (IGF)对体外培养兔关节软骨细胞存活数目和分裂增殖百分数的影响。方法 体外培养兔关节软骨细胞传至第 2代 ,分别以EGF、IGF ,以及二者不同的浓度组合作用于软骨细胞。通过酶联免疫检测仪 (MTT)测定软骨细胞存活数 ,流式细胞仪测定软骨细胞分裂增殖百分数。结果 不同浓度EGF对兔关节软骨细胞存活和分裂增殖均有促进作用 ,作用浓度强度次序为 :10ng/ml>0 1ng/ml>10 0ng/ml。不同浓度IGF对兔关节软骨细胞的存活和分裂增殖均有较强促进作用 ,浓度为 5 0ng/ml时 ,刺激效果最显著。EGF与IGF联合使用时 ,刺激效果优于任何一种因子单独使用 ,而以EGF 10ng/ml和IGF 5 0ng/ml为最佳浓度组合。 结论 EGF和IGF都可促进兔关节软骨细胞存活和分裂增殖 ,但以协同作用效果最佳。
Objective To study the influence of epidermal growth factor(EGF) and insulin-like growth factor(IGF) on the survival number and the splitting proliferation percentage of articular cartilage cells in rabbit in vitro Methods The cultured monolayer cells of articular chondrocytes in rabbit in vitro have been transferred to the second generation and the articular chondrocytes were treated with EGF or IGF,as well as the combinations of the both in various concentrations.The survival number of chondrocytes was investigated by MTT enzyme-linked immunometric meter and the splitting proliferation percentage of chondrocytes was determined by flow cytometer.Results EGF with various concentrations had auxo-action on the survival number and the splitting proliferation percentage of articular chondrocytes in rabbit.The intensity of the auxo-action decreases in the following order:10 ng/ml>0 1 ng/ml>100 ng/ml.IGF of various concentrations exhibited stronger auxo-action on the survival number and the splitting proliferation percentage of articular chondrocytes in rabbit than that of EGF.The most significant stimulation occurred at the concentration of 50 ng/ml.The cooperation of EGF and IGF was superior to either of the growth factors in stimulation and the best combining concentration was 10 ng/ml of EGF and 50 ng/ml of IGF.Conclusion It is shown that EGF and IGF are able to promote the survival number and the splitting proliferation percentage of articular chondrocytes in rabbit,but the cooperation of the both exhibits the best result.
出处
《中国骨伤》
CAS
2003年第1期23-25,共3页
China Journal of Orthopaedics and Traumatology
