摘要
背景:膝关节外伤可引起膝关节不稳定,其运动轨迹发生改变,继而可致关节软骨损伤,关节软骨中的主要成分软骨细胞、胶原纤维及蛋白多糖早期将发生变化。目的:比较研究两种家兔不稳定膝动物模型软骨退变进程中软骨细胞、胶原纤维、蛋白多糖变化,探讨早期不稳定膝关节软骨退变的发生机制。方法:取大耳白兔50只,随机分为3组前交叉韧带切除组、内侧副韧带+半月板切除组分别切除右后肢前交叉韧带、右后肢内侧副韧带和半月板,正常对照组不干预,分别在建模1,2,3,4和5周后分批于膝股骨内髁软骨取材,进行软骨组织大体和组织学观察、软骨损伤程度Markin评分;测定软骨组织蛋白多糖及软骨基质中胶原纤维含量。结果与结论:不同时期的不稳定膝关节软骨有程度不一的软骨细胞凋亡。除前交叉韧带切除组第1周外,各时间点两手术组Mankin评分均高于正常对照组(P<0.05),以术后5周组最为显著。前交叉韧带切除组术后3,4周软骨细胞变性,蛋白多糖丢失,术后第5周软骨退变更加显著,内侧副韧带+半月板切除组变化较前交叉韧带切除组严重。前交叉韧带切除组3,4,5周及内侧副韧带+半月板切除组2,3,4,5周软骨胶原纤维明显低于正常对照组(P<0.05)。结果表明,膝骨关节不稳定,早期关节软骨退变的程度与软骨细胞数目的减少、胶原纤维网的破坏和蛋白多糖的丢失有关,软骨退变过程中存在软骨修复。
BACKGROUND： Knee joint trauma results in knee instability and movement track changes, which lead to the degradation of articular cartilages. The main component of articular cartilages, such as chondrocytes, collagen and proteoglycan, are also change during this process. OBJECTIVE： To compare the content changes of collagen, proteoglycan and the apoptosis of chondrocytes on two model groups of knee joint instability, additionally, to investigate the occurrence mechanism of early articular cartilages changes in rabbits with knee joint instability. METHODS： A total of 50 white rabbits were randomly divided into anterior cruciate ligament transection group, medial collateral ligament transection ＋ meniscectomy group and normal control group. The specimens were harvested at weeks 1, 2, 3, 4, and 5 after operation. The osteocartilaginous section of the medial femur condyles were observed by gross observation, histological examination, and Markin scores. The contents of proteoglycan and collagen fiber were also measured. RESULTS AND CONCLUSION： Chondrocytes apoptosis were found with certain degrees at different stages of knee joint instability models. The Mankin score of the anterior cruciate ligament transection group was greater than the medial collateral ligament transection ＋ meniscectomy group at each time point except the 1st week （P 0.05）, which was notably greater in the 5th week. At weeks 3 and 4 after operation, the chondrocytes degenerated with proteoglycan lost in the anterior cruciate ligament transection group, and the chondrocytes degeneration was predominant at 5th week. The changes were more seriously in the medial collateral ligament transection ＋ meniscectomy group. Meantime, the collagen fiber content of two experimental groups were less than the normal control group at weeks 3, 4, 5 after operation （P 0.05）. The results demonstrated that at early knee joint instability, the articular cartilages degenerated extent is related to the number of chondrocytes, the collagen network disrupts and lost of proteoglycan. During chondrocytes degeneration, cartilage repair simultaneously occurred.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第7期1166-1169,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
