摘要
目的:研究大鼠股骨骨折合并脑外伤时骨痂组织中TGF-β1的表达及血清TGF-β1水平,探讨TGF-β1与骨折合并脑外伤时愈合加快的关系.方法:将64只大鼠随机分为4组∶正常对照组、脑外伤组、骨折组和骨折合并脑外伤组各16只.建立大鼠脑外伤和骨折模型,分别于2 wk和3 wk取血清,酶联免疫吸附法测血清中TGF-β1水平,骨折标本拍计算机X线摄像片,骨痂组织作HE染色和SP法免疫组化.结果:2 wk时大鼠血清TGF-β1水平在骨折合并脑外伤组(4.68±0.61)μg/L,脑外伤组(5.92±0.84)μg/L,骨折组(6.23±0.78)μg/L,与正常对照组(8.37±0.65)μg/L相比均有显著性降低(P<0.05);骨折合并脑外伤组与脑外伤组、骨折组比较有显著性降低(P<0.05);3 wk时各实验组大鼠血清TGF-β1水平有所恢复,骨折合并脑外伤组与正常对照组相比未见有统计学差异.骨痂组织TGF-β1免疫组化染色平均阳性细胞百分数骨折合并脑外伤组(85.2±9.2)%比骨折组(77.0±6.7)%有显著性增加(P<0.05).结论:伴有脑外伤的骨折愈合加快,TGF-β1可能是脑外伤后骨折愈合加速的因素之一.
AIM： To study the relationship between transforming growth factor-β1 （ TGF-β1 ） and enhanced osteogenesis after brain injury by investigating the expression of TGF-β1 in callus and the level of TGF-β1 in serum of rats with brain injury and femoral fracture. METHODS： 64 male SD rats were randomly divided into 4 groups： normal control, brain injury group, fracture group, fracture with brain injury group （n = 16 per group）. Fracture and brain injury models were established. The rats were sacrificed 2 and 3 weeks respectively after operation, and then the TGF-β1 concentration in serum was detected by rat TGF-β1 ELISA kit and the callus was examed with HE and SP immunohistochemical staining. RESULTS： The serum levels of TGF-β1 in the brain injury group [ （5.92 ±0.84）μg/L], fracture group [ （6.23±0.78 ） μ/L] or fracture with brain injury group [ （4, 68 ± 0.61 ） μ/L] significantly decreased as compared with that in control group [（8.37 ±0.65 ） μ/L, P 〈 0.05 ] at the 2nd week after operation, and recovered a little at the 3rd week after operation so that the difference between fracture with brain injure group and control group was not statistically significant. The concentration of the TGF-β1 in serum of the fracture with brain injury group was lower than that of brain injury or fracture group 2 weeks after operation （P 〈 0.05 ）. The mean percentage of positive cells in callus of the fracture with brain injury group [ （ 85.2 ±9.2 ） % ] was higher than that of the fracture without brain injury group [ （77.0 ± 6.7 ） %, P 〈 0.05 ]. CONCLUSION ： Fracture with brain injury had an enhanced healing response. TGF-β1 may play a role in enhanced osteogenesis after brain injury.
出处
《第四军医大学学报》
北大核心
2006年第15期1407-1410,共4页
Journal of the Fourth Military Medical University
关键词
转化生长因子
脑损伤
骨折
免疫组织化学
transforming growth factor
brain injury
fracture
immunohistochemistry
