摘要
介绍了水产品中氯霉素残留量测定的气相色谱法。样品中的氯霉素用乙酸乙酯提取,正己烷去脂肪,过Sep-C18柱进行净化,用BSTFA-TMCS衍生后进带有微电子俘获器的气相色谱仪检测。该方法的线性范围为0.5～500μg·L-1,相关系数r=0.999,最低检测限为0.1μg·kg-1,相对标准偏差为4.3%～11.0%,向样品中分别添加1μg·kg-1、10μg·kg-1和20μg·kg-13个浓度水平的氯霉素,回收率分别为62.0%、88.2%和96.4%。方法灵敏度高,准确可靠,适合水产品中氯霉素残留量的检测。
A gas chromatography method had been established for the determination of chloramphenicol in aquatic products. Chloramphenicol was extracted with ethyl acetate and partitioned with nhexane to remove lipids. Cleanup was performed on a Sep cartridge. After the drug was derived with BSTFA-TMCS,the derivatives were analyzed by capillary gas chromatography-electron capture detection. The linear range was from 0.5μg·L-1 to 500μg·L-1 with r of 0.999. The average recoveries were 62.0%, 88.2% and 96.4% respectively when samples were spiked with 1μg·kg-1, 10μg·kg-1 and 20μg·kg-1. The detection limit was 0.1μg·kg-1. The RSDs were 4.3%-11.0%. The method is sensitive, accurate and suitable for the determination of trace chloramphenicol in aquatic products.
出处
《水产学报》
CAS
CSCD
北大核心
2003年第3期278-282,共5页
Journal of Fisheries of China
基金
江苏省"十五"科技攻关资助项目(BE2001385)
