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酶联免疫(ELISA)法测定水产品中氯霉素残留量 被引量：1

The Application of ELISA Method for The Determination of Chloramphenicol(CAP) Residues in Aquatic Products
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摘要 建立水产品中氯霉素残留量的酶联免疫检测方法。比较不同样品处理方法对检测结果的影响 ,通过对检测范围、精密度、回收率等项目的测定 ,验证了方法的可靠性。氯霉素标准在 0 .0 5ng/ml～4 .0 5ng/ml范围内具有良好的线性 ,板间变异系数小于 16 .4 % ,样品测定的变异系数为 9.4 % ～13.8% ,乙腈水提取方法的回收率在 84 %～ 116 The ELISA method for the determination of chloramphenicol(CAP) residues in aquatic products has been studied.Different distillation method affected on the testing data.The standard curve was rectilinear from the rage of 0.05ng/ml to 4.05ng/ml.The intra-assay variations of kits was less than 16.4%. The variations of sample was 9.4%～13.8%. The recovery was 84%～116 %.
作者 黄冬梅 蔡友琼 毕士川 沈晓盛
机构地区 中国水产科学研究院东海水产研究所
出处 《福建水产》 2004年第4期20-23,共4页 Journal of Fujian Fisheries
关键词 ELISA 酶联免疫检测 检测结果 样品处理方法 水提 氯霉素残留量 样品测定 变异系数 水产品 提取方法 ELISA aquatic products chloramphenico
分类号 S859.84 [农业科学—临床兽医学] R446 [医药卫生—诊断学]
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共引文献63

同被引文献19

  • 1万善霞,徐修远,王建立,张淑萍.间接竞争ELISA检测兔肉中氯霉素残留方法的确定[J].动物科学与动物医学,2004,21(7):23-25. 被引量：1
  • 2魏书林,史为民,彭莉,沈建忠,原蕾,崔伟.鸡肌肉组织中氯霉素残留ELISA检测方法的研究[J].中国兽医杂志,2004,40(9):7-9. 被引量：9
  • 3罗杰,李健.呋喃唑酮间接竞争ELISA(ciELISA)检测法的建立[J].中国海洋大学学报（自然科学版）,2005,35(2):213-218. 被引量：42
  • 4安清聪,张曦,李琦华.四环素类抗生素残留的ELISA检测研究[J].食品科学,2005,26(6):205-207. 被引量：6
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  • 9Hiroo W,Atsuko S,Yasumasa K,et al.Mono- clonal-based enzyme-linked immunosorbent assay and immunochromatographic rapid assay for salinomycin[].Analytical Chimical.2001
  • 10Scortichini G,Annunziata L,Haouet M N,et al.ELISA qualitative screening of chloramphenicol in muscle,eggs,honey and milk:method validation ac- cording to the commission decision 2002/657/EC cri- teria[].Analytical Chimical.2005

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二级引证文献8

福建水产
2004年 第4期

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