摘要
目的 研究弓形虫速殖子各类抗原的蛋白组分 ,主要蛋白分子的抗原性和用于血清学诊断的价值。 方法 用聚丙烯酰胺凝胶电泳分析弓形虫速殖子各类抗原的蛋白组分和主要蛋白分子 ,用免疫印渍技术以弓形虫患者血清识别弓形虫各类抗原的免疫反应靶位。 结果 弓形虫速殖子细胞质抗原、细胞膜抗原和分泌代谢抗原的主要蛋白分子分别为 85、5 2、38ku;4 8、35、16、14 ku和 15 8、95、2 7、15、13ku及弓形虫患者特异性血清识别速殖子各类抗原的免疫反应靶位分别是 32、4 0和 2 7ku。 结论 弓形虫速殖子各类抗原间存在各自独特的抗原决定簇和可被特异性记忆抗体识别的免疫反应受体。此对于提高弓形虫感染的免疫学诊断及免疫学预防均具有一定的实用价值。
Objective To study the protein composition, molecular weight, major proteins molecular of Toxoplasma gondii antigens and analyze the specific immune reaction with serum of T. gondii patients. Methods Protein molecular of the cytoplasm extract(CE), cell membrane(CM) and excreted/secreted(ES) antigens were analyzed by SDS-PAGE. Using the protein gel transfer technique (western blotting) to recognize targets position of specific immune reaction of T. gondii various antigens with serum of toxoplasma patients, respectively. Results CE, CM and ES antigens of T. gondii major proteins molecular weight have 85, 52, 38, 32 ku; 48, 35, 16, 14 ku and 158, 95,27, 15, 13 ku respectively. The results also revealed that CE, CM and ES antigens were reacted with IgM and IgG in the serum of infected individuals of T. gondii on the 32 ku, 40 ku and 27 ku by western blotting respectively. Conclusion The various antigens of T. gondii hold a specific antigenic determinant and specific immune response receptors that it can be recognized by specific antibody. This results may possess important value to explore immunodiagnosis and immunoprophylaxis.
出处
《中国寄生虫病防治杂志》
CSCD
2003年第3期144-146,共3页
Chinese Journal of Parasitic Disease Control
基金
贵州省科学技术研究基金 (No.2 0 0 0 .30 2 7)。
