摘要
目的观察快动眼睡眠剥夺大鼠下丘脑神经元型一氧化氮合酶 (nNOS )及诱导型一氧化氮合酶(iNOS )mRNA表达的时相变化。方法小站台水环境法剥夺大鼠睡眠 2 4h、48h及 72h后 ,用半定量逆转录聚合酶链反应 (RT -PCR )检测其下丘脑nNOS及iNOSmRNA的表达。结果睡眠剥夺 2 4h组nNOSmRNA表达量显著增加 (P <0 .0 1 ) ,剥夺 48h组与对照组比较无显著差异 ,剥夺 72h组nNOSmRNA的表达量低于对照组水平 (P <0 .0 5 ) ;睡眠剥夺 2 4h和 48h组iNOSmRNA的表达量无显著变化 ,但剥夺 72h组iNOSmRNA的表达量大幅度增加 (P <0 .0 1 )。结论睡眠剥夺后NOS基因表达增加 ,可能是睡眠“反跳”现象的机理之一 ;
Objective To study the effects of rapid eye movement sleep ( REMS ) deprivation on the expression of mRNA coding for neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) of hypothalamus in rats. Method Flower pot technique was adopted to deprive the REMS of Sprague Dawley rats for 24 h, 48 h and 72 h respectively. The expression of mRNA coding for nNOS or iNOS of hypothalamus in rats was assayed by reverse transcription polymerase chain reaction ( RT PCR ). Result The amount of nNOS mRNA was significantly higher in 24 h REMS deprivation group ( P<0.01 ), then the amount was lowered in 48 h deprivation group and became significantly lower in 72 h deprivation group than that in the control group (P<0.05 ). There was low expression of iNOS mRNA of hypothalamus in rats, and there was no difference in the expression of iNOS mRNA among 24 h, 48 h REMS deprivation and the control groups. But the expression was significantly increased in 72 h REMS deprivation group ( P<0.01 ). Conclusion Deprivation of REMS increased the expression of nNOS and iNOS mRNA of hypothalamus. Excessive nitric oxide ( NO ) might be a major factor resulting in not only the sleep rebound phenomenon but also the injury of human function caused by sleep loss directly or indirectly by other sleep factors.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2003年第3期175-178,共4页
Space Medicine & Medical Engineering
基金
全军"九五"医学科研规划指令性课题资助项目( 96L0 3 8)
