摘要
目的 :用高效液相色谱法测定苦甘颗粒中黄芩苷的含量。方法 :色谱柱为ODSC18,流动相为甲醇 -水 -醋酸 (45∶55∶1) ,检测波长为274nm。结果 :黄芩苷在0 25μg～2 5μg范围内进样量与峰面积呈现良好的线性关系(r=0 9992) ;平均加样回收率为98 40 % ,RSD=1 09% (n=5)。结论 :本方法测定结果准确 。
OBJECTIVE:To develop a HPLC method for determination of the content of baicalin in Kugan granˉules.METHODS:ODS-C 18 column was used and methanol-water-acetic acid(45∶55∶1)was used as mobile phase and UV detection wavelength was274nm.RESULTS:There was a good linear relationship between the concentration of Baicalin and absorption area value in range of0.25μg～2.5μg r=0.9992.The average recovery was98.4%with RSD=1.09%(n=5). CONCLUSION:The method was highly repeatable and accurate.
出处
《中国药房》
CAS
CSCD
2003年第6期366-367,共2页
China Pharmacy
