摘要
目的 :应用高效液色相谱法测定清热灵颗粒中黄芩苷的含量。方法 :色谱柱为Alltech:AlltimaC1 8(5μm 4 6× 1 50mm) ,流动相为甲醇 水 冰醋酸 (50∶50∶1 ) ,,检测波长为 2 74mm ,流速为 1 0ml/min。结果 :黄芩苷 0 1 0 36μg～0 82 88μg范围内进行样量与峰面积呈良好线性关系 (r=0 9995) ;平均加样回收率 98 84% ,RSD =1 44% (n =5)。结论 :本方法测定结果准确、灵敏。
Objective:To develop an HPLC method for determination of the content of baicalin in Qingreling granules.Methods:Alltima C 18 column was used and methanol water acetic acid glacial(50∶50∶1) was used as mobile phase and UV detection wavelength was 274nm. Results: There was a good linear relationship between the concentration of Baicalin and absorption area value in range of 0 1036ug～0 8288ug,r=0 9995.The average recovery rate was 98 84% with RSD=1 44%(n=5).Conclusion:The method was highly repeatable and accurate.
出处
《浙江中医学院学报》
2004年第3期62-63,共2页
Journal of Zhejiang College of Traditional Chinese Medicine
