摘要
采用天然高分子絮凝剂壳聚糖对L 异亮氨酸发酵液进行预处理,在发酵液pH2、壳聚糖用量30mg/L的条件下可取得较好的絮凝效果.通过静态吸附实验,考察了pH和L 异亮氨酸质量浓度对平衡吸附量的影响,最后确定了732#离子交换树脂提取L 异亮氨酸的最佳工艺条件:上柱发酵液pH2,上柱速度0.6BV/h,洗脱液为0.5mol/L的NH4Cl,洗脱体积流量0.5BV/h.洗脱液经脱色、浓缩结晶后得L 异亮氨酸成品,总提取率为55%.
Pretreatment of Lisoleucine fermentation broth was conducted by using chitosan. Excellent coagulation result was achieved under the following conditions: pH 2.0 and chitosan dosage 30 mg/L. Effects of factors such as pH and concentration of Lisoleucine on absorption capacity were investigated by static state absorption experiment. The optimal operation conditions of extracting Lisoleucine were as following: fermentation broth of pH 2, sample flowrate of 0.6 BV/h, 0.5 mol/L NH4Cl as the eluant and the elution velocity of 0.5 BV/h. The effluent was then decolored, concentrated and crystallized. The total recovery ratio is 55%.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2003年第3期76-79,共4页
Journal of Wuxi University of Light Industry
