摘要
目的 探讨上海地区汉族人群载脂蛋白 E( apolipoprotein E,apo E)基因启动子区 - 4 2 7C/ T多态性与 Alzheimer病 ( Alzheimer's disease,AD)发病风险的关系。方法 采用聚合酶链反应和限制性片段长度多态性方法 ,在 10 4例 AD患者和 110名正常人中检测了 apo E基因 - 4 2 7C/ T各基因型及基因频率的分布。按比值比 ( odds ratio,OR)作疾病关联分析。结果 ( 1) AD患者与正常对照人群之间不存在- 4 2 7C/ T各等位基因和基因型频率分布的差异 ( P>0 .0 5 ) ;( 2 )按 apo Eε4基因分层后 ,无论是 ε4型人群还是非 ε4人群都不存在 AD患者与正常老人间多态分布的差异 ( P>0 .0 5 ) ;( 3)在 - 4 2 7C/ T3种基因型中 ,仅T/ T型 AD与 apo Eε4等位基因呈正关联 ( OR=3.94 ,95 % CI:2 .2 0 6～ 7.0 38,χ2 =2 1.4 8,P<0 .0 5 )。结论上海地区汉族人群中 ,apo E基因 - 4 2 7C/ T多态不是 AD的疾病易感因子。
Objective: To determine the relation between the apolipoprotein E(apoE) promoter -427C/T polymorphism and Alzheimer's disease (AD) in a Chinese Han population in Shanghai. Methods: The apo E promoter -427C/T polymorphism in 104 AD cases and 110 healthy subjects was detected using polymerase chain reaction method and restriction fragment length polymorphism genotyping technique. The differences in polymorphic distribution between the two groups were tested, and odds ratio was computed. Results: No differences in apoE -427C/T genotypic distribution were observed between AD cases and controls (P > 0. 05). Even after stratification according to apoE Ε4 stratum, there was not any polymorphic distribution difference when Ε4 carriers or non-Ε4 carriers were compared with controls (P> 0. 05). The association between AD and apoE Ε4 appeared in the TT group(OR = 3.94, 95% CI: 2. 206-7. 038, χ2 = 21. 48, P < 0.05), but not in CT or CC group. Conclusion: ApoE -427C/T polymorphism was not a susceptibility factor for AD in this Han population in Shanghai.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
2003年第3期244-246,共3页
Chinese Journal of Medical Genetics
基金
上海市卫生局青年课题资助项目 (994Y1 2)~~
