摘要
从阿魏侧耳 (Pleurotusferulae)子实体中用热水浸提出粗多糖PFW ,将PFW脱蛋白后 ,经DEAE—纤维素柱层析 ,得两种多糖PF1 和PF2 。用光散射和GPC联机测定 :PF1 含有两种组分其重均分子量分别为 7 875× 1 0 5 和 3 2 4 5× 1 0 4 ;PF2 重均分子量为 3 1 87× 1 0 6。用IR和GC分析它们组成和结构 ,结果表明 :PF1 由鼠李糖、葡萄糖、半乳糖和甘露糖组成 ,含有 β 糖苷键和甘露糖苷 ;PF2 由鼠李糖、木糖、葡萄糖、半乳糖和甘露糖组成 ,含有α 糖苷键。给小鼠腹腔注射多糖 ,对移植性肿瘤S 1
PFW was isolated from fruit body of Pleurotus ferulae by using hot water Proteins were removed from PFW with Sevag method, after DEAE cellulose chromatography, PF 1 and PF 2 were isolated from PFW The weight was determined by using multiangle laser photometer combined with GPC; the results showed: PF 1 contains two fractions, the weights (Mw) are 7 875×10 5 and 3 245×10 4, the Mw of PF 2 is 3 187×10 6 The components and structure of polysaccharides were analyzed by IR and GC The polysaccharides PF 1 consists of rhamnose, glucose, galactose, mannose; PF 2 consists of rhamnose, xylose, glucose, galactose, mannose; PF 1 contains β glucoside and mannoside, PF 2 contains α glucoside It was found polysaccharides have antitumor activity on the implanted Sarcoma 180 in mice
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第2期16-19,共4页
Microbiology China
基金
湖北省重点科技计划资助项目 (No 95 2P1 0 0 7)
关键词
阿魏侧耳
多糖
分离纯化
抗肿瘤活性
Pleurotus ferulae , Polysaccharides, Isolation, Purification, Antitumor activity
